重庆青蒿中青蒿素含量及其AFLP分析

Artemisinin Contents and AFLP Analysis of Artemisia annua Grown in Chongqing of Southwest China

本文对重庆地区酉阳县、沙坪坝和北碚的10个主要青蒿类型进行了青蒿素含量测定及其AFLP分析。研究结果表明:不同的青蒿株系青蒿素含量不同,栽培株系和色茎株系青蒿素含量较高;进一步的AFLP分析表明引物组合EA04-MC05和EA01-MC01分别在高产青蒿株系和茎色青蒿株系中均能扩增出一条特异性条带;根据AFLP差异带,用WPGMA法对10个主要青蒿类型进行聚类分析,结果没有得到与青蒿素含量的明确关联,只是从产地上将酉阳青蒿与北碚、沙坪坝青蒿区分,说明不同地区青蒿的遗传差异较大。本文通过研究影响青蒿素含量的环境因素和遗传因素,为进一步克隆青蒿素合成相关基因提供参考。

In this research,we significantly investigated 10 major kinds of southernwoods which were grown in Youyang county,Shapingba and Beipo were determined the artemisinin contents as well as the AFLP analysis of them.The results showed that different southernwood cultivars displayed different artemisinin contents,especially,the artemisinin contents of planting cultivars and the cultivars with coloured stem were higher.Further AFLP analysis indicated that a specific fragment was amplified respectively with the primers of EA04-MC05 and EA01-MC01.According to the differentia fragments,we clustering analyzed the 10 major kinds of southernwoods by utilizing WPGMA approach.However,there was no distinctly relativity between the artemisinin contents and diversity of AFLP analysis and just can distinguish,southernwood of Youyang county from Shapingba and Beipo in producing area which still illuminated that the heredity differentia of southernwood presented itself obviously in different area.Therefore,via studying on the environmental and genetic factors of affecting the artemisinin content,we believed that it would provide the reference for further cloning some correlation gene of artemisinin synthesis.