贵州毛南族人群CSF1PO,TPOX和TH01STR基因座基因频率调查

Investigation on gene frequencies of CSF1PO,TPOX and TH01STR loci in Maonan ethnic group in Guizhou

目的：调查贵州毛南族人群CSF1PO,TPOX和TH01STR基因座等位基因频率.方法：chelex-100提取法提取104名毛南族无关个体DNA,PCR复合扩增,变性PAGE分型,直接计数基因型,用modified-powerstats软件包计算各多态性指标,采用SPSS13.0统计软件包对贵州毛南族人群与广西两个毛南族群体相同基因座等位基因频率进行比较.结果：CSF1PO,TPOX和TH01STR基因座共检出等位基因数17个,等位基因频率范围在0.0288~0.4087之间;共检出基因型40种,各基因座基因型分布符合Hardy-Weinberg平衡定律（P〉0.05）.3个基因座的杂合率（H）、非父排除率（PPE）、个体识别率（DP）、多态信息量（PIC）分别大于0.69,0.5,0.8,0.6,累积个体识别率（TDP）为0.99743.贵州毛南族人群和广西两个毛南族群体等位基因频率分布有统计学意义（P〈0.05或P〈0.01）.结论：CSF1PO,TPOX和TH01 3个STR基因座在贵州毛南族人群中有较高的遗传多态性,在群体遗传学和法医鉴定中有较高的应用价值.起源于古代同一族系的同一民族或不同民族,遗传结构有丰富的遗传多样性,其等位基因频率分布与地域呈平行关系.

AIM：To investigate gene frequencies of CSF1PO,TPOX and TH01STR loci in maonan ethnic Group in Guizhou province.METHODS：With chelex-100 method,the DNA of 104 unrelated Maonan individuals were extracted,the DNA samles were multiple amplified and PCR products were typed using denaturated PAGE techniques.Using modified-powerstats and SPSS13.0 software package,the polymorphism data was caculated and comparison the gene frequencies between Maonan in Guizhou and two Maonan populations in Guangxi in same STR loci.RESULTS：A total of 17 alleles and 40 genotypes were detected in 3 STR loci,the range of allele gene frequecies from 0.0288 to 0.4087.The distribution of genotypes met Hardy-Weinberg equilibrium（P〉0.05）.The heterozygosities（H） 〉0.69,probabilities of paternity exclusion（PPE）〉0.5,discrimination power（DP）〉0.8,polymorphism information content（PIC）〉0.6 in CSF1PO,TPOX and TH01STR loci respcetively and total of discrimination power（TDP） of those of STR was 0.99743.There was statistical sence of the distribution of allele gene frequecies between Maonan in Guizhou and Guangxi（P〈0.05 or P〈0.01）.CONCLUSION：The results indicate that there are high genetic polymorphism in Guizhou Maonan and high application value in population genetic and forensic research.There was genetic diversity in same or different ethnic groups in different region that there were same origin and there was parallel relation between the distribution of allece gene frequencis and their living region.