p16,p53和Mad2在胃癌中的表达及临床病理学意义

Expression of p16,p53 and Mad2 in gastric cancer and its clinicopathological significance

目的:研究p16,p53和Mad2基因在胃癌中的表达及其临床病理学意义.方法:应用免疫组化SP法检测胃癌组和对照组(正常胃粘膜、慢性浅表性胃炎、慢性萎缩性胃炎、增生性息肉、不典型增生)中p16,p53和Mad2蛋白的表达.结果:对照组中p16,p53和Mad2的阳性率分别为74.6%,32.8%和59.7%,而胃癌中p16,p53和Mad2的阳性率分别为46.3%,58.7%和79.1%,各指标在两组间的表达均有显著性差异(P<0.05);p16在累及浆膜组的表达与未累及浆膜组间有显著性差异(P<0.05);p16,p53和Mad2在有淋巴结转移组与无淋巴结转移组间均存在显著性差异(P<0.05).结论:p16,p53和Mad2基因表达的异常在胃癌的发生、发展和预后中起着重要的作用.

AIM：To study the p16,p53 and Mad2 gene expression in gastric cancer and its clinicopathological significance.METHODS：In gastric cancer group and control group（normal gastric mucosa,chronic superficial gastritis,chronic atrophic gastritis,hyperplastic polyp,atypical hyperplasia）,detect the p16,p53 and Mad2 protein expression with SP immunohistochemical method.RESULTS：The p16,p53 and Mad2 positive rates were 74.6%,32.8% and 59.7%in the control group and 46.3%,58.7% and 79.1% in the gastric cancer group,the indicators in the two groups expression were significantly different（P〈0.05）;p16 expression was a significant difference（P〈0.05） in serosa involved group and not serosa involved group;p16,p53 and Mad2 in the group with lymph node metastasis and lymph node metastasis group there are significant differences（P〈0.05）.CONCLUSION：The p16,p53 and Mad2 gene abnormal expression play an important role in gastric cancer incidence,development and prognosis.