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siRNA干扰对Eca-109食管癌细胞株Skp2表达的影响

Effect of small interference RNA on expression of the Skp2 in Eca-109 cell line
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摘要 目的:探讨siRNA干扰后食管癌Eca-109细胞株Skp2 mRNA表达及肿瘤细胞增殖活性的影响.方法:通过siRNA转染食管癌Eca-109细胞株后,分别用RT-PCR和CCK-8检测Skp2 mRNA的表达和细胞增殖的活性.结果:食管癌Eca-109细胞株增殖活性及Skp2 mRNA明显减少.结论:运用siRNA可以有效抑制Skp2 mRNA的表达及细胞增殖活性.siRNA有望成为治疗食管癌的重要手段. AIM：To study the expression of Skp2 mRNA and the proliferation activity of Eca-109 cell line after transfected Skp2 siRNA.METHODS：The Skp2 siRNA chain were synthesized and transfected into Eca-109 cell line by RNA-Mate reagent.RT-PCR was used to analyzed the quantity of Skp2 mRNA,CCK-8 was used to detect the proliferation activity of Eca-109 cell line.RESULTS：Significant down-regulation of the Skp2 mRNA were found following transfection of the Skp2 siRNA chain,and the proliferation activity of Eca-109 cell line was inhibited.CONCLUSION：The transfection of synthesized Skp2 siRNA in Eca-109 cell line can down-regulate the expression of Skp2 mRNA.As an important tool of the gene silencing siRNA is possibly to be a new method of treating the esophageal cancer.
作者 宋旭东 徐洪
机构地区 华北煤炭医学院病理学教研室
出处 《第四军医大学学报》 北大核心 2009年第23期2791-2793,共3页 Journal of the Fourth Military Medical University
关键词 SKP2 小干扰RNA 食管癌 Skp2 siRNA esophageal cancer
分类号 R735.1 [医药卫生—肿瘤]
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参考文献13

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第四军医大学学报
2009年 第23期

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