荧光分光光度法测定巴戟天中蒽醌的含量

Spectrofluorimetric determination of anthraquinone content in Morinda Officinalis How

目的：建立荧光分光光度法测定中药巴戟天中蒽醌含量的方法,并初步比较不同产地巴戟天中蒽醌之间的含量差异性.方法：以丙酮为溶剂,1,8-二羟基蒽醌为对照品,在最大激发波长为425 nm,发射波长为513 nm处测定对照品及样品溶液的荧光强度.结果：在247.5~2970μg/L范围内,1,8-二羟基蒽醌溶液浓度与荧光强度呈现良好的线性关系（r=0.9995）;不同产地巴戟天的游离蒽醌含量范围为29.26~69.95μg/g,结合蒽醌含量为3714.59~5830.39μg/g.结论：荧光分光光度法测定巴戟天中蒽醌的含量,方法可靠、重复性好;不同产地巴戟天中游离蒽醌、结合蒽醌的含量有一定差异.

AIM：To develop the spectrofluorimetric method to determine the anthraquinone content of Morinda Officinalis How,and to discuss the relations of anthraquinone content of Morinda Officinalis in different metropolis preliminarily.METHODS：1,8-dihydroxyanthraquinone was reference substance.Take acetone as solvent agent,the fluorescent intensity of the solution was measured at 425/513 nm.RESULTS：The linear range was 247.5-2970 μg/L（r=0.9995）.Free anthraquinone and combined anthraquinone content of Morinda Officinalis in different growing areas range were 29.26-69.95 μg/g,3714.59-5830.39 μg/g.CONCLUSION：This research for measuring anthraquinone content in Morinda Officinalis provides a kind of effectively dependable analytical method.Free anthraquinone and combined anthraquinone compositions of Morinda Officinalis in different metropolis have some differences.