血管内皮生长因子基因防治兔髂动脉狭窄

Vascular endothelial growth gene therapy for narrowing of rabbit iliac artery

目的从基因治疗方面探讨解决经皮血管成型术和其他血管再塑引起的血管内膜损伤，血管狭窄途径。方法构建了ｐｃＤＮＡ３／血管内皮生长因子（ＶＥＧＦ）真核表达载体，通过多聚赖氨酸基因球囊法，将ＶＥＧＦ基因定位导入球囊拉伤的兔髂动脉内壁，应用原位杂交，免疫组织化学，电磁血流量仪，扫描电镜和光镜等方法检测ＶＥＧＦ基因作用效果。结果ＶＥＧＦ基因组球囊拉伤血管２周时内膜有大量ＶＥＧＦｍＲＮＡ和蛋白表达，内膜内皮化范围明显大于空载质粒对照组，内膜与中膜面积之比小于对照组，血流量明显改善。结论血管内导入ＶＥＧＦ基因，可促进内皮细胞增生，加速损伤内膜的修复，抑制血管内膜平滑肌细胞的增生。

Objective To study the mechanism of renarrowing and vascular endothelial growth factor (VEGF) gene therapy to prevent the restenosis caused by balloon angioplasty. Methods According to the special function of VEGF that promepts the proliferation of endothelial cell (EC), we constituted an eukaryotic expression vector of pcDNA 3/VEGF, and added the pcDNA 3/VEGF to the surface of polylysine balloon. We examined the results of the test by in situ blot, eletromagnetic blood flow mechine, scanning eletromicroscope, and light microscope. Results There was higher expression of VEGF mRNA and iliac artery intima in the VEGF gene group. The extent of endothelialization was larger, the ratio of intima to media (I/M) was smaller, and the blood flow was more sufficient than in the vector control group. Conclusion VEGF gene local delivery could stimulate the proliferation of EC, accelerate the endothelialization of injury artery, and inhibite the proliferation of smooth muscle cell (SMC).