转染P^(53)基因对胶质瘤细胞株SHG44裸鼠致癌性的作用研究

Study on the role of P^(53) gene transfer on human glioma cell growth in nude mice

探讨转染野生型P^(53)（wt一P^(53)）和突变型P^(53)（mtP^(53)）基因，对人胶质瘤细胞株SHG44裸鼠致瘤性的影响及意义。方法：采用质脂体介导法，分别将wt一P^(53)和mt一P^(53)基因导入人胶质瘤细胞株SHG44，体内外检测转导细胞的生长状况和裸鼠致瘤性。结果：转染mt一P^(53)基因的细胞株，G418筛选细胞集落数、软琼脂平皿细胞集落数以及裸鼠瘤组织重量和体积，均显著高于对照组（P＜0．01）；而转染Wt一P^(53)基因的细胞株均显著低于对照组（P＜0．01），表明导入wt一P^(53)基因的细胞株，瘤细胞生长速度明显低于对照细胞株和导入。mt一P53基因的细胞株，即导入mt一P^(53)基因的细胞株瘤细胞生长速度最快，而导入Wt-P^(53)基因的细胞株瘤细胞生长速度最慢。结论：Wt一P^(53)基因能有效地抑制人胶质瘤细胞生长；mt一P^(53)基因则可以明显地促进瘤细胞生长。

Objective To explore the significance and role of P^(53) gene transfer on human glioma cell growth in nude mice. Method Wild-type P53 gene (wt-^(53)) and mutant P53 gene (mt-^(53)) were transfected into the human glioma cell line SHG44. And the growth of gene-transfected cell lines were observed in vitro and in vivo. Results The tumor resulting from the cells transfected with the wt-P53 gene more slowly and was smaller than that from control SHG44 cells. In contrast, the tumor from the cells transfected with the mt-P^(53) gene grew faster than that produced by cells transfected with the Wt-P^(53) gene and that produced by control SHG44 cells. Conclusion The wild-typeP^(53) gene could inhibit the glioma cell growth in nude mice and the mutant P^(53) gene could enhance the glioma cell growth in nude face.