胰岛内分泌肿瘤中α-HCG蛋白及mRNA表达的研究──应用免疫组化及原位PCR技术对52例的观察

Study on express of α-HCG and α-HCGmRNA in pancreatic endocrine tumors using immunohistochemistry and in situ PCR

应用免疫组化对52例胰岛内分泌肿瘤（PET）α－HCG蛋白表达进行观察；并用原位PCR技术中对其α-HCGmRNA的表达进行观察。结果发现α-HCG在各类正常胰岛细胞中未见蛋白及基因的阳性表达。在良性及恶性PET中α-HCG蛋白检出率分别为17．9％（5／28）及58．3％（14／24）。在α－HCG抗原阳性的PET中应用原位PCR技术均可见有α－HCGmRNA的表达。说明该类肿瘤细胞具有合成α-HCG的能力。对α－HCG在PET中表达的临床病理意义进行了研讨。

The antigent AlPha-subunit of human chorionic gonadompin(α-HCG) and α-HCGmRNA in pacreatic endocrine tumors(PET) were investigated by immunohistochemistry and in situ PCR. It was found that α-HCG and α-HCGmRNA can be detected in PET buy not in normal islet cells. α-HCG immunoreactivity was expressed by 5 of 28(17 .9% ) in benign PET and 14 of 24(58. 3% ) in malignant PET. Using in situ PCR of α-HCGmRNA, a strong signal in PET was obtained. The clinicopathological sighficance of α-HCG in PET was discussed.