CCR7在多器官功能障碍综合征小鼠脾脏树突状细胞迁移变化中的作用

CCR7 implications of spleen dendritic cells in multiple organ dysfunction syndrome in mice

目的：探讨CCR7在多器官功能障碍综合征（MODS）脾脏中的表达变化及其对树突状细胞（DC）迁移的影响。方法：用酵母多糖腹腔注射复制小鼠MODS模型,分为正常对照组和实验3-6小时组、24-48小时组、5-7天组及10-12天组。运用免疫组化方法检测CD11c和CD205标记阳性DC在各组小鼠脾脏中分布的变化,用流式细胞术检测CD86/CD11c和CCR7/CD11c标记阳性细胞在脾脏中含量的变化。结果：正常小鼠脾脏DC含量较少,主要分布在脾脏边缘区;在3-6小时组CCR7表达率较正常对照组显著增加,DC含量显著增加、活性增高,并向白髓T细胞区大量迁移;24-48小时组T细胞区中DC含量开始减少,而CCR7表达率升高达到峰值;5-7天组DC与CCR7含量接近正常对照组,边缘区和T细胞区均可见DC分布;10-12天组DC含量再次升高,但多呈不成熟状态,且以边缘区分布为主,CCR7表达率下降。结论：在MODS病程中脾脏DC的含量和分布变化与CCR7的表达率密切相关,CCR7可以作为评估脾脏DC迁移能力及功能活性的重要指标。

Objective： To explore CCR7 expression in splenic dendritic cells and its role in migration and activity of splenic dendritic cells in multiple organ dysfunction syndrome （MODS） in mice. Methods： The MODS model of mice was reproduced by Zymosan injection into peritoneal cavity. The mice were randomly divided into groups of normal, 3-6 hours, 24-48 hours and 10-12 days post zymosan injection. CD1 l c and CD205 were analysed by immunohistochemistry; The expression of CD86 and CCR7 of DCs were studied by the flow cytometry analysis.Results：In normal mice,many DC were found at the margin between the red and white pulp.In the 3-6 h and 24-48 h greups, CD86 and CCR7 were strongly up-regulated in the DC, and they distributed in T cells areas. In the 10-12 d group, DC distributed at the margin by the immature form. Conclusion： The CCR7 expression level of DC has close correlations with the migration of DC, CCR7 can be used to evaluate the migration and functional activity of DC in MODS.