慢性肝病患者外周血Ghrelin与炎性细胞因子的水平检测及其相关性研究

Detections and relevance of Ghrelin and inflammatory cytokines in peripheral blood of patients with chronic hepatic diseases

目的:探讨外周血Ghrelin与炎性细胞因子TNF-αI、L-1及IL-6表达水平与慢性乙型肝炎、肝炎肝硬化、非酒精性脂肪性肝病肝脏损害程度的相关关系。方法:慢性肝病患者组和健康对照组血浆中Ghrelin、TNF-αI、L-1及IL-6表达水平检测采用酶联免疫吸附实验(ELISA)。结果:慢性乙型肝炎、肝炎肝硬化患者外周血Ghrelin表达明显升高,与正常对照组比较差异显著(P<0.05,P<0.01),失代偿期肝硬化(B、C级)患者外周血Ghrelin水平明显高于慢性乙型肝炎组(P<0.05);Ghrelin在非酒精性脂肪肝病患者组外周血明显降低,与正常对照组比较差异显著(P<0.01);慢性肝炎、肝炎肝硬化患者外周血Ghrelin表达与炎性细胞因子TNF-αI、L-1及IL-6呈正相关(慢性肝炎组r值分别为0.587、0.995、0.985,肝硬化组r值分别为0.730、0.966、0.979),而在NAFLD疾病组则呈负相关(r值分别为-0.660、-0.873、-0.894)。结论:Ghrelin在慢性乙型肝炎、肝炎肝硬化患者组外周血高表达,而在非酒精性脂肪性肝病患者组外周血低表达,Ghrelin表达水平与慢性肝病的发生发展过程相关。

Objective：The purpose of this study is to determine levels of Ghrelin, TNF-α, IL-I and IL-6 in peripheral blood of patients with chronic hepatitis B, liver cirrhosis, and non-alcoholic fatty liver disease which diagnosed at different extent of damage, and based on it to have a further study on the relevance between Ghrelin and inflammatory cytokines in peripheral blood in above diseases. Methods： Ghrelin, TNF-α, IL-1 and IL-6 in peripheral blood were determined by ELISA method. Results： In patients with chronic hepatitis B andhepatic cirrhosis, Ghrelin level increased significantly as compared with that in normal control group （ P 〈 0. 05, P 〈 0.01 ）. Ghrelin levels in decompensated liver cirrhosis （degree B and C）groups were much higher than in chronic hepatitis B group （ P 〈 0.05）. Ghrelin level in group of non-alcoholic fatty liver disease decreased significantly as compared with that in normal control group （P 〈 0.01）. Ghrelin and inflammatory cytokines, including TNF-α, IL-1 and IL-6 showed a positive relevances in groups of chronic hepatitis B and hver cirrhosis, but not in NAFLD group which showed a negative correlations. Conclusion： Ghrelin has a higher level in peripheral blood of patients with chronic hepatitis B and liver cirrhosis,but it has a lower level in patients with non-alcoholic fatty liver disease,suggesting correlation of Ghrelin level with the occurrence and the procession of chronic liver diseass.