自体纯化CD34^+细胞移植治疗特发性嗜酸性粒细胞增多症的临床研究

Transplantation of highly purified autologous CD34^+ cells from peripheral blood for treatment of HES

目的总结国内首例自体纯化CD34+细胞移植治疗特发性嗜酸性粒细胞增多症的经验。方法对1例36岁特发性嗜酸性粒细胞增多症男性患者实施自体纯化CD34+细胞移植,观察造血重建及移植相关并发症,探索该治疗手段的可行性。结果移植后患者症状体征消失,外周血嗜酸性粒细胞计数恢复正常;中性粒细胞大于0.5×109/L,血小板大于20×109/L的时间都为+12d;+45d内未观察到肝静脉闭塞病、肝肾功能损伤等移植并发症。结论自体纯化CD34+细胞移植为治疗特发性嗜酸性粒细胞增多症的有效手段。

Objective To explore the clinical effect and the safety for treatment of hypereosinophilia syndrome（HE5） by trans plantation of highly purified autologous peripheral blood CD34^＋ cells. Methods A 36 years male patient,with HSE,was treated with transplantation of highly purified autologous peripheral blood CD34^＋ cells in Xinqiao Hospital. Peripheral blood stem ceils （ PBSCs ） were mobilized with G-CSF alone,CD34^＋ cells were enriched by CliniMACS. The high dose regimen was FAM conditioning regimen （fludarabine, mitoxantrone, cytosine arabinoside） and we observed the hematopoietic reconstitution and complications correlated with AHSCT. Results The purity and recovery of the CD34 ^＋ cell product were 99 %, the mediumterm for neutrophil re covering to 0.5×10^9/L and platelet 20×10^9/L existed in 12d and 14d post transplant,without serious toxic reaction and complication found. Conclusion The transplantation of highly purified autologous CD34^＋ cells from peripheral blood is effective and safe for HES.