乳酸杆菌对宫颈肿瘤细胞的黏附抑制作用

Lactobacillus’ sticky and depressant effect on uterine cervix cancer cells

目的探讨乳酸杆菌对宫颈肿瘤细胞的黏附与黏附抑制作用。方法采取30例妇女宫颈脱落上皮细胞,与乳酸杆菌震荡培养1-3h、经革兰染色观察宫颈脱落上皮细胞上乳酸杆菌的黏附指数。取对数生长宫颈癌Hela细胞与乳酸杆菌共同培养1-72h,观察不同时间段宫颈癌细胞乳酸杆菌的黏附指数以及细胞活力变化。结果1）乳酸杆菌对宫颈脱落上皮细胞有很好的黏附作用：在培养2h黏附指数达高峰（活菌组39.44±2.5;灭活组85.1±2.9）;灭活组黏附指数在共培养2、3h均明显高于活菌组（P〉0.01）。2）乳酸杆菌对宫颈癌细胞的黏附作用开始随着培养时间增加逐渐增加,两组黏附指数在共培养12h达高峰（活菌黏附指数为25.69±3.1;灭活菌黏附指数为90.49±2.6）,之后逐渐降低。3）在共培养开始短时间内乳酸杆菌并不影响细胞活力;活菌组共培养12h、灭活组共培养24h宫颈癌细胞活力开始下降。结论乳酸杆菌能很好的黏附并抑制宫颈癌细胞的生长;灭活乳酸杆菌对宫颈细胞以及宫颈癌细胞有着更好的黏附抑制作用。

Objective To explore lactobacillus＇ sticky and depressant effect on uterine cervix cancer cells. Methods 1. Cervix uteri amotic epithelialis cells from 30 women were shake cultured with lactobacillus 1-3h, and the lactobacillus＇ sticky index number on cervix uteri amotic epithelialis ceils was observed through Gram＇ s stain. 2. Uterine cervix cancer ceils （Hela cells） and lactobacillus were co-cultured 1-72h, and the lactobacillus＇ sticky index number on cervix uteri cancer cells and cell activity changes at different times were observed. Results 1. Lactobacillus had good sticky effect on cervix uteri amotic epithelialis cells： There appeared sticky index number climax （viable lactobacillus 39.44 ± 2.5; inactivation lactobacillus 85.1 ± 2.9） 2h after the co-culture began. Inactivation lactobacillus had better sticky effect on cervix uteri amotic cellula epithelialis, and the sticky index number of inactivation lactobacillus was higher than that of viable lactobacillus 2-3h after the co-culture began （P〈0.01）. 2. The sticky effect of lactobacillus on uterine cervix cancer cells increased gradually with the culture time. There appeared sticky climax （viable lactobacillus 25.69 ± 3.1; inactivation lactobacillus 90.49 ± 2.6） 12h after the co-culture began; but after that, the sticky index number decreased gradually. 3. At the beginning of the co-culture, lactobacillus did not affect the activity of uterine cervix cancer cells. In viable lactobacillus, the activity of uterine cervix cancer cells began to decrease 12h after the co-culture began; and in inactivation lactobacillus, the activity of uterine cervix cancer cells began to decrease 24h after the co-culture began. Conclusions Lactobacillus could stick and protect cervix uteri cellula epithelialis and prevent paraplasm of cervix uteri cellula epithelialis. Lactobacillus could also stick and restrain uterine cervix cancer cells. Thus inactivation lactobacillus had better sticky and depressant effect on uterine cervix cells and uterine cervix cancer cells.