狗牙根辐射诱变后代变异植株的形态特征比较和ISSR分析

Analysis on the morphological characters and ISSR of the variant plant of Cynodon dactylon

对狗牙根Cynodon dactylon‘Princess.77’辐射诱变后代中筛选的7个变异植株与其对照以及目前草坪工程中应用的‘Common’、‘Jackpot’、‘Ulma’、‘Nume×Sahara’、‘Tifway’、‘Tifgreen’等共14份材料进行表型性状比较和ISSR分析,结果表明：各变异植株与对照之间在节间长度方面均有显著差异;节间直径除HN016与对照差异不显著外,其他变异植株与对照之间差异均显著（P〈0.05）;变异植株HN015的绿期最长,平均270.3 d,HN010的绿期最短,平均242.3 d;从38个ISSR随机引物中筛选出10个引物,对这14个狗牙根材料的基因组DNA进行PCR扩增,并进行遗传相似系数与聚类分析,形态变异植株与其对照之间的遗传相似系数为0.585-0.776,遗传差异性比较明显。

The morphological characters and ISSR of 14 materials,including variants,control and other cultivars of Cynodon dactylon,were analyzed.The results indicated that the difference of internode length was significant between variants and the control,and the difference of internode diameter were also significant except HN016.The green period of HN015 was longest and HN010 was shortest.Ten primers were screened from 38 arbitrary primers of ISSR,14 genomic DNA of C.dactylon materials were amplified,the results showed that the genetic similarity was from 0.585 to 0.776 between variants and the control and their genetic variability was remarkable.