槐花中抗氧化及清除自由基活性物质的提取条件研究

Optimization of extraction conditions for antioxidant and free radical scavenging substance production from pogadatree flower

主要探讨不同提取条件对槐花提取物清除自由基能力的影响。结果表明,70%乙醇槐花提取液的清除自由基活性物质、总酚和总黄酮的含量最高;清除超氧阴离子能力最强的提取温度为45℃,其余均为50℃;提取物总抗氧化能力、清除DPPH自由基和羟自由基能力最强的料液比是1∶20,其余则是1∶15;槐花经过两次提取可获得83%～95%的清除自由基活性物质、总酚和总黄酮。槐花提取物中总抗氧化能力和清除DPPH自由基能力与酚类和黄酮类物质具有较强的相关性。

The present study was to optimize the extraction conditions that give maximum free radical scavenging substances from pogadatree flower （Sophora japonica L.）. The results showed that the pogadatree flower extraction with 70% ethanol provided the highest free radical scavenging substance, total phenol and total flavonoids content.The optimal extraction temperature for superoxide anion scavenging substance was identified as 45℃,and other free radical scavenging substances were determined as 50℃.The rate of material and solvent for most efficient extraction of total antioxidant activity, DPPH, hydroxyl radical-scavenging substance was 1：20, and the remainders was 1：15.Twice extraction of pogadatree flower showed about 83%-95% of the total free radical scavenging substance, phenol and flavonoids content. The results of correlation analysis showed that total antioxidant activity and DPPH free radical mainly came from phenol and flavonoids.