汉防己甲素对慢性缺氧大鼠肺胶原的影响

Effect of tetrandrine on the collagen contents of lungs in rats with chronic hypoxia

目的探讨汉防己甲素（Ｔｅｔ）对慢性低氧性肺动脉高压大鼠肺组织胶原含量的影响。方法雄性大鼠３０只，随机分为：（１）对照组１０只，不缺氧处理；（２）单纯缺氧组１０只，每只于低氧前后腹腔注射生理盐水１ｍｌ，共２１天，每天缺氧８小时；（３）汉防己甲素处理组１０只，于每天缺氧前后按７５ｍｇ·ｋｇ１腹腔注射汉防己甲素，共２１天。应用氯胺Ｔ氧化比色法测定肺组织和肺外肺动脉的羟脯氨酸（Ｈｙｐ）含量和马三（ｍａｓｏｎ）胶原特殊染色法，图像分析技术及灰度扫描观察低氧引起肺组织和肺外肺动脉胶原含量的变化及汉防己甲素处理后的影响。结果（１）慢性低氧使大鼠的平均肺动脉压（ｍＰＡＰ）、肺循环阻力（ＰＶＲ）及心输出量（ＣＯ）与正常对照组比较，差异有显著性（Ｐ＜０．０１），而汉防己甲素处理组的ｍＰＡＰ、ＰＶＲ与单纯缺氧组比较差异有显著性（Ｐ＜０．０５）；（２）慢性低氧大鼠肺外动脉羟脯氨酸含量为１８３±１２μｇ·ｇ１，与正常对照组的１０６±１０μｇ·ｇ１比较差异有显著性（Ｐ＜０．０１），经汉防己甲素处理后降为１３９±１５μｇ·ｇ１，与单纯缺氧组比较差异有显著性（Ｐ＜０．０１），但与正常对照组比较亦有差异（Ｐ＜０．０５）；（３）单纯?

Objective To investigate the effect of tetrandrine (Tet) on the collagen contents of lung tissues in rats with chronic hypoxic pulmonary hypertension. Method Using colorimetric method after oxidized by chloramin T to determine hydroxyproline contents of lung tissues and extrapulmonary arteries, using Masson method, image pattern analysis technique and gray scale scanning to observe the changes of collagen distribution in extrapulmonary arteries and lung tissues in rats with hypoxia and the effect of treatment with Tet. Result Tet could remarkably reduce the mean pulmonary arterial pressure and pulmonary vascular resistance in rats with hypoxic pulmonary hypertension and could inhibite the increase of collagen contents in lung tissues and extrapulmonary arterial wall. Conclusions Tet may be used as one of the main drugs in the treatment of chronic hypoxic pulmonary hypertension due to its action of reducing mPAP and collagen contents.