摘要
目的探讨多形核白细胞(PMN)变形性变化在大鼠内毒素性急性肺损伤(ALI)中的作用机制及山莨菪碱对PMN变形性变化的影响。方法44只大鼠通过静脉注射内毒素(5mg/kg)复制大鼠ALI模型,每组分为:(1)内毒素1小时组(8只);(2)内毒素4小时组(8只);(3)内毒素6小时组(8只);(4)山莨菪碱治疗组(8只),注射内毒素后,立即静脉注射山莨菪碱5mg/kg;(5)正常对照组(12只),用等量生理盐水替代内毒素。用微管吸吮技术检测大鼠ALI过程中PMN变形性变化。并用光镜观察肺组织病理变化。结果PMN的弹性模量(K1)于静脉注射内毒素后1小时(33±12Pa,1Pa=0.0075mmHg),4小时(37±12Pa)和6小时(38±13Pa)与对照组(26±9Pa)比较差异有显著性(P<0.01),PMN的粘性模量(μ)于静脉注射内毒素后4小时(5±3Pa.s)和6小时(6±3Pa.s)与对照组(31±26Pa.s)比较,差异有显著性(P<0.01);PMN弹性剪切模量(G)于静脉注射内毒素后4小时(54±20Pa)和6小时(56±21Pa)与对照组(47±17Pa)比较,(P<0.01),山莨菪碱?
Objective To exlore the mechanisms of change of deformability of polymorphonuclear leukocytes (PMN) and influence of anisodamine on deformability of PMN during endotoxin induced acute lung injury (ALI)in rat. To explore the role of PMN in ALI. Methode The model of ALI in rat was reproduced by intravenous injection of E Coli endotoxin.The deformability of PMN was measured by micropipette aspiration technique. Result The elastic modulus K 1 of PMN at the 1st hour (33±12 Pa),4th hours (37±12 Pa, 1 Pa=0.0075 mmHg) and 6th hours (38±13 Pa) after injection of E Coli endotoxin are all significantly higher than that in the control group (26±9 Pa P <0.01). The viscosity modulus (μ) of PMN at the 4th hours (5±3 Pa.s) and 6th hours (6±3 Pa.s)after injection of E Coli endotoxin are all significantly higher than the control group (3.1± 2.6 Pa.s, P <0.01). The elastic shear modulus (G) of PMN at the 4th hours (54±20 Pa) and the 6th hours (56±21 Pa) after injection of E Coli endotoxin are all significantly higher than the control group (47±17 Pa, P < 0.05). The K 1 (33±12 Pa) and the μ(4±3 Pa.s) in anisodamine group are all significantly lower than endotoxin group in the same time phases ( P <0.05). Conclusion The deformability of PMN decrease remarkably during ALI, which will enhance PMN sequestration in lungs, lead to ALI. It may be one of importent pathogenic mechanism of ALI. The anisodamine can inhibit the decrease of deformability of PMN effectively and may have some therapeutic effcet on ALI.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1998年第10期598-600,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases