急性肺损伤大鼠白细胞变形性变化及山莨菪碱影响

Changes of the deformability of neutrophil and the influence of anisodamine on neutrophil deformability during endotoxin induced acute lung injury in rats

目的探讨多形核白细胞（ＰＭＮ）变形性变化在大鼠内毒素性急性肺损伤（ＡＬＩ）中的作用机制及山莨菪碱对ＰＭＮ变形性变化的影响。方法４４只大鼠通过静脉注射内毒素（５ｍｇ／ｋｇ）复制大鼠ＡＬＩ模型，每组分为：（１）内毒素１小时组（８只）；（２）内毒素４小时组（８只）；（３）内毒素６小时组（８只）；（４）山莨菪碱治疗组（８只），注射内毒素后，立即静脉注射山莨菪碱５ｍｇ／ｋｇ；（５）正常对照组（１２只），用等量生理盐水替代内毒素。用微管吸吮技术检测大鼠ＡＬＩ过程中ＰＭＮ变形性变化。并用光镜观察肺组织病理变化。结果ＰＭＮ的弹性模量（Ｋ１）于静脉注射内毒素后１小时（３３±１２Ｐａ，１Ｐａ＝０．００７５ｍｍＨｇ），４小时（３７±１２Ｐａ）和６小时（３８±１３Ｐａ）与对照组（２６±９Ｐａ）比较差异有显著性（Ｐ＜０．０１），ＰＭＮ的粘性模量（μ）于静脉注射内毒素后４小时（５±３Ｐａ．ｓ）和６小时（６±３Ｐａ．ｓ）与对照组（３１±２６Ｐａ．ｓ）比较，差异有显著性（Ｐ＜０．０１）；ＰＭＮ弹性剪切模量（Ｇ）于静脉注射内毒素后４小时（５４±２０Ｐａ）和６小时（５６±２１Ｐａ）与对照组（４７±１７Ｐａ）比较，（Ｐ＜０．０１），山莨菪碱?

Objective To exlore the mechanisms of change of deformability of polymorphonuclear leukocytes (PMN) and influence of anisodamine on deformability of PMN during endotoxin induced acute lung injury (ALI)in rat. To explore the role of PMN in ALI. Methode The model of ALI in rat was reproduced by intravenous injection of E Coli endotoxin.The deformability of PMN was measured by micropipette aspiration technique. Result The elastic modulus K 1 of PMN at the 1st hour (33±12 Pa),4th hours (37±12 Pa, 1 Pa=0.0075 mmHg) and 6th hours (38±13 Pa) after injection of E Coli endotoxin are all significantly higher than that in the control group (26±9 Pa P <0.01). The viscosity modulus (μ) of PMN at the 4th hours (5±3 Pa.s) and 6th hours (6±3 Pa.s)after injection of E Coli endotoxin are all significantly higher than the control group (3.1± 2.6 Pa.s, P <0.01). The elastic shear modulus (G) of PMN at the 4th hours (54±20 Pa) and the 6th hours (56±21 Pa) after injection of E Coli endotoxin are all significantly higher than the control group (47±17 Pa, P < 0.05). The K 1 (33±12 Pa) and the μ(4±3 Pa.s) in anisodamine group are all significantly lower than endotoxin group in the same time phases ( P <0.05). Conclusion The deformability of PMN decrease remarkably during ALI, which will enhance PMN sequestration in lungs, lead to ALI. It may be one of importent pathogenic mechanism of ALI. The anisodamine can inhibit the decrease of deformability of PMN effectively and may have some therapeutic effcet on ALI.