小肠缺血再灌注后不同时限小肠黏膜细胞凋亡变化的研究

Research on Dynamic Change of Epithelial Cells Apoptosis in the Rats Model of Intestinal Ischemia-Reperfusion Injury

目的探讨小肠缺血再灌注后不同时限小肠黏膜细胞凋亡的动态变化。方法选用健康雄性SD大鼠,建立小肠缺血再灌注损伤模型,分为缺血再灌注组与对照组。每组大鼠根据缺血再灌注后不同时限,分为对照、缺血再灌注后O′,30′,1h,2h,3h,6h,12h,24h,48h,72h,96h共12组(每组6只)。在缺血再灌注后不同时间点,采用HE染色观察小肠黏膜组织学形态,TUNEL染色检测小肠黏膜凋亡细胞。结果大鼠小肠历经热缺血40min后,再灌注0～96h大致经历了潜伏期、急性期、交错期、恢复期4个阶段的形态变化。缺血再灌注后,出现明显的细胞凋亡及组织学病理改变,开始于潜伏期,加重于急性期。结论小肠在短时间缺血后、再灌注可出现明显的细胞凋亡及组织学病理改变。大多数小肠黏膜细胞经历短暂的凋亡前期反应后则进入恢复阶段,最终结果是小肠黏膜结构和功能的修复。

Objective To provide experiment evidence for treating the intestine damage induced by ischemia-reperfusion in intestinal surgical operation in rats. Methods Intestinal isehemia-reperfusion injury models were established in SD rats. According to different ischemie and reperfusive time,the rats were divided into ischemia-reperfusion groups and control groups. The reperfusion was performed 40 minutes after ischemia in the intestines of the SD rats. The intestinal tissues of the experimental groups were taken at 0min ,30min, 1 h ,2h ,3h, 6h, 12h,24h,48h,72h and 96h respectively after the reperfusion and the control groups were treated with pseudo-operation. The mucosa epithelial cells apoptosis and proliferation induced by ischemia-reperfusion were dynamically determined by TUNEL. Results The rats＇intestines underwent 4 stage changes as following, incubation period, acute phase, overlap stage and recovery stage from 40 minutes after ischemia to 96 hours after reperfusion. The epithelial cells apoptosis and histopathologic change induced by ischemia-reperfusion began at the incubation stage,aggravated at the acute stage. Conclusions This is an obviously epithelial cells apoptosis and histopathologie changes after a short time isehemia and reperfusion. Most cells turned into convalescence after a short pre-apoptosis reaction, then the intestine mucosal structure and function were repaired.