人乳头瘤病毒16E6蛋白、细胞周期素D_1及端粒酶逆转录酶在鼻咽癌组织中的表达及意义

Expression of E6 oncoprotein of HPV16,cyclin D_1,and human telomerase transcriptase in nasopharyngeal carcinoma tissues and its significance

目的:探讨人乳头瘤病毒(HPV)16早期编码蛋白E6、细胞周期素D1(cyclin D1)及人端粒酶逆转录酶(human telom-erase transcriptase,hTERT)在鼻咽癌发生发展中的作用及临床意义。方法:采用免疫组织化学SP法检测HPV16E6、cyclinD1、hTERT在鼻咽癌组织和鼻咽部慢性炎症组织中的表达情况,分析其与鼻咽癌临床病理参数间的关系及其对预后的影响。结果:HPV16E6、cyclin D1、hTERT在鼻咽癌组织中阳性表达率分别为62.5%(35/56)、50.0%(28/56)、67.9%(38/56),明显高于鼻咽部慢性炎症组(P<0.05)。三者阳性表达率随组织学分级的降低、临床分期的增加及淋巴结转移而升高(P<0.05),与患者年龄及性别无关(P>0.05)。HPV16E6分别与cyclin D1和hTERT呈正相关(r=0.480,P<0.001;r=0.494,P<0.001)。HPV16E6、cyclin D1和hTERT阳性患者平均生存期、中位生存期均低于阴性患者(P<0.05)。结论:HPV16E6、cyc-lin D1、hTERT的高表达可能与鼻咽癌的发生及发展相关。HPV16E6与cyclin D1,HPV16E6与hTERT之间可能存在相互作用;同时检测这3个指标可能对判断鼻咽癌患者预后有指导意义。

Objective：To evaluate the role of HPV16E6,cyclin D1,and human telomerase transcriptase（hTERT） in the development and progression of nasopharyngeal carcinoma（NPC） and to discuss the clinical significance.Methods： Immunohistochemistry was used to detect the expression of HPV16E6,cyclin D1,and hTERT in paraffin-embedded nasopharyngeal carcinoma tissues and nasopharyngeal chronic inflammation tissues.The relationship between their expression with the clinicopathological features of NPC was analyzed;the influence of their expression on prognoses of patients was also analyzed.Results： The positive rates of HPV16E6,cyclin D1,and hTERT in NPC tissues were 62.5%（35/56）,50.0%（28/56）,and 67.9%（38/56）,respectively,which were significantly higher than those in the inflammation tissues（P〈0.05）.The positive rates of HPV16E6,cyclin D1,and hTERT increased with the decrease of differentiation grades,increase of clinical stage and presence of lymph node metastases（P〈0.05）,but had no relationship with patients＇ age or sex（P〉0.05）.HPV16E6 expression was positively correlated with cyclin D1（r=0.480,P〈0.001） and hTERT（r=0.494,P〈0.001）in NPC tissues.The mean survival period and median survival period in HPV16E6,cyclin D1 and hTERT positive patients were lower than those in the negative ones（P〈0.05）.Conclusion： High expression of HPV16E6,cyclin D1,and hTERT might be involved in the development and progression of NPC.HPV16E6 may interact with cyclin D1 and hTERT,contributing to the development of NPC.Examination of the three agents may help to predict the prognoses of NPC.