垂体腺苷酸环化酶激活多肽调节LPS激活的树突状细胞免疫功能

Pituitary adenylate cyclase-activating polypeptide modulates immune function of LPS-stimulatd dendritic cells

目的:研究垂体腺苷酸环化酶激活多肽(pituitary adenylate cyclase-activating polypeptide,PACAP)能否调节小鼠骨髓起源的LPS激活的树突状细胞(dendritic cell,DC)的免疫功能。方法:联合应用rmGM-CSF和rmIL-4自C57BL/6小鼠骨髓细胞制备DC,以LPS和(或)PACAP刺激DC;收集被激活的DC及其上清液行ELISA和流式细胞术分析;自DC提取总RNA行RT-PCR和RNase分析。结果:PACAP能明显抑制LPS激活的DC分泌细胞因子IL-2、IL-12和TNF-α(P<0.05,P<0.01,P<0.01),以及LPS激活的DC分泌趋化因子MIP-2(P<0.01),但抑制细胞因子IL-6,趋化因子MIP-1α和MIP-1β的作用并不明显(P>0.05)。结论:PACAP对LPS激活的DC的免疫功能具有负性调节作用。

Objective：To investigate whether pituitary adenylate cyclase-activating polypeptide（PACAP）can modulate the immune function of mouse LPS-stimulated dendritic cells.Methods： DCs were derived from bone marrow of C57BL/6 using rmGM-CSF and rmIL-4,then the DCs were exposed to different stimuli for indicated time periods.The stimulated DCs and their culture supernatant were subjected to ELISA and FACS analysis.The total RNA was extracted from the stimulated DC for RT-PCR and RNase Protection Assay.Results： PACAP significantly inhibited the production of IL-2,IL-12,TNF-α and MIP-2 in LPS-stimulated DCs（P〈0.05,P〈0.01,P〈0.01,P〈0.01,respectirely）,but the inhibition of LPS-induced IL-6,MIP-1α and MIP-1β production was not apparent.Conclusion： PACAP can negatively modulate the immunity of LPS-stimulated DCs.