超重环境对斑马鱼仔鱼早期发育和耳石形态学与化学组成的影响

Influences of Hyper-gravity on Early Development,Otolith Morphology and Otolith Chemical Compositions of Zebrafish Larva,Danio Rerio

目的本研究以模式动物斑马鱼为研究对象,通过研究超重下鱼体的早期发育和耳石变化,探讨了脊椎动物前庭系统对超重环境的适应机制。方法斑马鱼受精卵在受精后10h分别置于1G和5G重力环境中,观察鱼体发育状态和耳石的形态;使用Zeiss软件比较1G和5G下耳石大小;使用EDAX检测耳石微化学成分含量变化。结果1)斑马鱼从鱼卵向早期仔鱼发育过程中超重环境影响仔鱼发育,耳石形态在初期显著变大(P<0.01),其后耳石变小;2)EDAX检测表明,耳石中S和Sr含量升高,Na和Ca的含量减少;3)出现矢耳石与微耳石融合的现象。结论超重环境影响斑马鱼早期发育,耳石的形态与微化学组成发生变化,导致耳石的功能发生变化。

Objective To study zebrafish, as model vertebrate, on its early development and otolith changes under hypergravity, trying to disclose the adaptation mechanism of vestibular system of vertebrate animal to hypergravity. Methods Zabrafish zygotes were put into 1 G and 5 G instruments respectively. Their development status and otolith morphology were observed. Otolith size was measured with the software of Zeiss. Otolith trace elements were examined with EDAX. Results 1 ） Hypergravity influenced early development of zebrafish from zygote to early larva, and otolith size significantly larger in the early stage （P 〈 0.01 ） but decreased subsequently. 2） Examined with EDAX, the contents of sulfur and Sr increased while Ca and Na contents decreased. 3） It was observed that sagitta and lapilli otoliths were fused into one otolith. Conclusion Hypergravity influences early development of zebrafish and causes changes of otolith size, morphology and microchemistry, resulting in the change of otolith function.