组织芯片检测β-连环蛋白、c-erbB2在胃腺癌中的表达及其临床意义

Detection of Expressions and Clinical Significances of β-catenin and c-erbB2 in Gastric Adenocarcinoma by Tissue Microarray

背景:近年来组织芯片已越来越多地应用于恶性肿瘤的相关研究,但用于胃癌研究的报道较少。目的:探讨胃腺癌、癌旁组织和外周正常黏膜组织中β-连环蛋白和c-erbB2的表达及其临床意义。方法:取102例胃腺癌、97例癌旁组织和98例外周正常黏膜组织分别制成含282点、156点和156点的3块组织芯片。以免疫组化法检测组织芯片中β-连环蛋白和c-erbB2的表达,并分析其与胃腺癌临床病理特征的关系。结果:胃腺癌组织中β-连环蛋白和c-erbB2阳性表达率显著高于癌旁组织和外周正常黏膜组织(57.0%对22.1%和7.3%,P=0.000;29.0%对5.3%和0%,P=0.000)。β-连环蛋白和c-erbB2异常表达与胃腺癌的分化程度(P=0.027,P=0.001)和浸润深度(P=0.033,P=0.011)有关,与其他临床病理特征无关。胃腺癌中β-连环蛋白表达与c-erbB2表达呈明显正相关(P<0.05)。结论:c-erbB2异常表达可能导致靶基因β-连环蛋白异常表达,从而在胃腺癌的发生、发展中起重要作用。

Background： Tissue microarray has been used more frequently in the relevant study of cancer in recent years, but its use in the study of gastric adenocarcinoma is still quite few. Aims： To study the expressions and clinical significances of β-catenin and c-erbB2 in gastric adenocarcinoma, para-caneerous tissue and normal mucosal tissue. Methods： One hundred and two specimens of gastric adenocarcinoma, 97 para-eancerous tissues and 98 normal mucosal tissues were collected and made into three pieces of tissue microarray containing 282 dots, 156 dots and 156 dots, respectively. Expressions of β-catenin and c-erbB2 were determined by immunohistoehemical staining, and their correlation with clinicopathological characteristics of gastric adenocarcinoma were analyzed. Results： The positivity rates of expressions of β-catenin and c-erbB2 in gastric adenocareinoma were significantly higher than those in para-eancerous tissue and normal mucosal tissue （57.0% vs. 22.1% and 7.3%, P=0.000; 29.0% vs. 5.3% and 0%, P=0.000）. Expressions of β-eatenin and c- erbB2 were correlated with cell differentiation （P=0.027, P=0.001） and depth of invasion （P=0.033, P=0.011）, but no correlation was seen with other clinicopathological characteristics. The expression of β-eatenin was significantly correlated with the expression of e-erbB2 in gastric adenocarcinoma （P〈0.05）. Conclusions： Abnormal expression of c-erbB2 may activate the expression of target gene β-catenin, which plays a pivotal role in the development and progression of gastric adenoearcinoma.