C-反应蛋白影响单核细胞趋化能力的研究

Effects of C-reactive protein on chemotaxis ability of monocytes in vitro

目的通过检测不同质量浓度C-反应蛋白(CRP)对体外培养的人单核细胞趋化能力的影响,探讨CRP作为炎症介质联系牙周炎与冠心病的分子机制。方法采用不同质量浓度CRP作用于体外培养的THP-1单核细胞,采用Transwell趋化实验检测单核细胞被趋化因子单核细胞趋化蛋白-1(MCP-1)趋化的数量。结果Transwell趋化实验发现,CRP作用于THP-1单核细胞后可增强其趋化能力。CRP质量浓度为2μg·mL-1时,单核细胞的趋化数量上调(P<0.05);随着CRP质量浓度升高,趋化的单核细胞增多(P<0.05)。结论CRP能增强THP-1单核细胞的趋化能力,且呈剂量依赖性。

Objective To investigate the effects of C-reactive protein （CRP） on monocytes chemotaxis ability in vitro. Methods Transwell chemotaxis assay was used to evaluate the changes of chemotactic ability of THP-1 monocytes in each group treated with CRP in different concentration. Results CRP increased the number of attracted monocytes in response to MCP-1 （monocyte cbemoattractant protein-1）. When treated with CRP concentration at 2 μg·mL^-1, the number of chemotactic monocytes increased （P〈0.05）. The number of attracted monocytes increased as CRP concentration was elevated （P〈0.05）. Conclusion CRP can increase chemotactic ability of THP-1 monocytes in concentration dependent manner.