小鼠骨髓间充质干细胞对同种异体骨髓来源的树突状细胞分化和功能的影响

The effect of murine mesenchymal stem cells on the differentiation and function of allogenetic bone marrow-derived dendritic cells

目的:研究小鼠骨髓间充质干细胞(BMSCs)对同种异体骨髓来源的树突状细胞(DCs)分化、成熟及功能的影响,探讨MSCs发挥免疫调节作用的机制。方法:体外分离培养BALB/c小鼠BMSCs,与C57BL/6小鼠骨髓有核细胞(BMCs)在体外按不同的细胞比例混合培养,诱导DC分化,流式细胞术(FCM)分析DC细胞表型及FITC-Dextran内吞能力,ELISA检测分泌的细胞因子IL-12的水平。结果:当MSC/BMC达到较高的比例(1∶10)时,细胞表面的CD11c、CD14、CD83、CD86、I-Ab的表达均显著下降(P<0.05),FITC-Dextran内吞能力及IL-12分泌水平亦显著降低(P<0.05)。结论:小鼠MSCs在体外能抑制同种异体骨髓来源的DCs的分化、成熟。

AIM： To study the effect of murine mesenchymal stem cells （MSCs） on the differentiation, maturation and function of allogenetic bone marrow-derived dendritic cells （DCs） and to investigate the mechanism of MSCs displaying immunoregulatory activity. METHODS： BALB/c mice BMCs were isolated and cultured in vitro and then coclutured with C57BL/6 murine bone marrows cells （BMCs） at different ratios in vitro to induce DCs generation. The phenotype of cells was analyzed by flow cytometry. Endocytosis was measured as the cellular uptake of fluorescein isothiocyanate （FITC） -dextram amd was quantified by flow cytometry. The level of IL-12 secretion in the cell culture supernatants was detected by enzyme-linked immunosorbent assay （ELISA）. RESULTS： Decreased expression of CD11c, CD14, CD83, CD86 and I-Ab, down-regulated endocytosis capacity and interleukin-12 （IL-12） secretion of DCs were all observed when MSCs cocultured with BMCs at a higher ratio （1：10）. CONCLUSION： Murine MSCs could supress the differentiation and maturation of DCs derived from allogeneic BMCs in vitro.