地塞米松对哮喘小鼠肺组织中转录因子RORγt mRNA表达的干预作用

Effects of dexamethasone on expression of Th17 transcription factor RORγt mRNA in experimental asthma mice

目的:初步探讨地塞米松对哮喘小鼠模型肺组织中Th17细胞转录因子维甲酸相关孤儿受体γt(RORγt)mRNA表达的影响。方法:采用卵清蛋白致敏方法建立支气管哮喘小鼠模型,BALB/c小鼠30只随机分为正常对照组、哮喘组、地塞米松治疗组各10只。采用酶联免疫吸附试验(ELISA)技术检测小鼠肺泡灌洗液(BALF)、血清中白细胞介素-17(IL-17)水平;无创肺功能体描仪检测小鼠气道反应性;HE染色评价各组小鼠气道炎症程度;逆转录聚合酶链反应(RT-PCR)方法检测肺组织IL-17、RORγt mRNA表达水平。结果:哮喘组小鼠肺组织RORγt mRNA、IL-17水平高于对照组(P<0.01),地塞米松治疗组RORγt mRNA、IL-17水平低于哮喘组(P<0.05)。结论:地塞米松可抑制哮喘小鼠肺组织RORγt表达,阻止Th17的分化,从而减少IL-17的分泌,减轻哮喘气道炎症反应。

AIM： To study the effects of dexamethasone （Dex） on expression of Th17 transcription factor retinoic acid-related orphan receptor gamma t （RORyt） in asthma. METHODS： The BALB/c mice asthma model was induced by ovalbumin（OVA） with classic method. Thirty female mice were randomly divided into control group, asthmatic group and Dex treated group. The level of IL-17 in mice bronchoalveolar lavage fluid （BALF） and serum was measured by enzyme-linked immunosorbent assay（ELISA）. Airway Responsiveness to acetylcholine chloride（Ach） was measured by a modified non-invasive method; The airway inflammation was evaluated by HE staining. The expression of RORyt mRNA was measured by reverse transcription-polymerase chain reaction（RT-PCR）. RESULTS： The level of RORyt mRNA, IL-17 of asthmatic group were significantly higher than those of control group（ P〈0.01）, which were significantly reduced by Dex compared with the asthmatic group（ P 〈 0.05）. CONCLUSION： Dex can inhibit the release of IL-17, whose mechanism may be the blockade of TH17 differentiation in asthmatic models by inhibit the RORyt expression.