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Claudin 18在胰腺癌细胞中的表达 被引量：2

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摘要 目的:检测人胰腺癌细胞中Claudin 18的表达,为临床诊断胰腺癌提供新的标志物和治疗靶位。方法:采用RT-PCR、免疫印迹和流式细胞术分别从基因、蛋白和细胞整体水平上检测胰腺导管腺癌和胰岛细胞癌细胞表面人源性Claudin 18的mRNA转录和蛋白的表达。结果:胰腺导管腺癌PANC-1、BX-PC-3和AS-PC-1细胞均可以在mRNA、蛋白和细胞整体的不同水平表达Claudin 18;而胰岛细胞癌RINm5F细胞则不表达Claudin 18。同时,对照细胞胃印戒细胞癌KATOIII也可以大量表达Claudin 18。结论:多数胰腺导管腺癌细胞系均可以在不同水平表达Claudin 18,而胰岛细胞癌细胞系则不能表达Claudin 18,该结果与前期胰腺癌患者的免疫组化检测结果一致。
作者 曹云新 张松 杨安钢
机构地区 第四军医大学免疫学教研室 第四军医大学药物研究所
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2009年第12期1202-1203,1206,共3页 Chinese Journal of Cellular and Molecular Immunology
关键词 Claudin18 胰腺导管腺癌 标志物
分类号 R392.11 [医药卫生—免疫学]
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共引文献33

同被引文献17

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细胞与分子免疫学杂志
2009年 第12期

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