摘要
目的从T淋巴细胞分化的多环节观察儿童自身免疫性甲状腺疾病Graves病(GD)和桥本甲状腺炎(HT)Th1/Th2类细胞的极化偏移方向,探讨其免疫学发病机制。方法采用ELISA和RT-PCR法同步检测GD(46例)和HT(64例)患儿的转录因子、细胞因子以及游离CD30(sCD30)水平,另设24例健康体检儿为对照组。结果GD、HT患儿外周血单个核细胞(PBMC)转录因子GATA-3的表达较正常组明显降低,T-bet、T-bet/GATA-3比值明显升高。GD、HT患儿PBMC产生IFN-γ水平和IFN-γ/IL-4比值较正常组明显增加,而IL-4水平明显减少。GD、HT患儿sCD30水平高于正常对照组。结论GD及HT患儿的前体T细胞具有向Th1分化发育的趋向。
Objective To study the relationship of transcription factors, cytokines, sCD30, and the polarization of Th1/Th2 cells in children with Hashimoto＇s thyroiditis （HT） and Graves disease （GD）. Methods Transcription factor, eytokine, and sCD30 levels were evaluated by ELISA and RT-PCR in children with GD and HT. Results The expression of transcription factor GATA-3 in peripheral blood mononuclear ceils （PBMC） was significantly lower, while T-bet, T-bet/GATA-3 ratio increased significantly in children with GD and HT than those in normal controls. IFN-γ and the ratio of IFN-γ/IL-4 levels in PBMC was significantly higher, while IL-4 level was significantly lower in children with GD and HT than those in normal controls. The sCD30 level was higher in children with GD and HT than that in normal controls. Conclusions This study suggested that TH0 cells showed trends of Thl differentiation in patients with HT and GD.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2009年第12期1111-1114,共4页
Journal of Clinical Pediatrics
基金
重庆市卫生局科研项目[No.渝卫科教(2003)61号(03-2-033)]
