非小细胞肺癌中PTEN及p53的表达及其临床意义

Expression of PTEN and p53 proteins and its clinical significance in non-small cell lung carcinoma

目的研究非小细胞肺癌(NSCLC)中PTEN及p53的表达及其临床意义。方法采用免疫组化S-P法,对61例NSCLC手术切除标本进行PTEN及突变型p53蛋白表达研究,分析其与NSCLC患者的病理类型及临床分期、预后的关系。结果(1)非小细胞肺癌中PTEN的表达明显低于对照组(P<0.05);而p53蛋白则明显增高。(2)PTEN表达与组织学类型、临床分期和淋巴结转移显著相关;p53表达与性别和淋巴结转移显著相关。(3)非小细胞肺癌标本中PTEN与p53的表达呈显著负相关(rs=-0.282,P<0.05)。(4)Kaplan-Meier生存分析显示,PTEN表达阴性组患者平均生存期为36.5月,阳性组为71.0月;p53表达阴性组患者平均生存期为70.1月,阳性组为46.0月,经Logrank检验均显示P<0.05。结论PTEN的表达缺失及野生型p53基因的突变,可能是导致NSCLC发生的重要原因之一,可以作为肿瘤基因治疗的靶点;PTEN及p53的表达与NSCLC患者的预后关系密切,可能是肿瘤预后的标志物;PTEN与p53的表达呈明显的负相关,两者是否通过同一途径,或者两条途径如何相互影响,需进一步研究。

Objective To investigate the expression of PTEN and p53 proteins and its clinical significance in non-small cell lung carcinoma （NSCLC）. Methods The expression of PTEN and mutated p53 was determined using immunohistochemistry S-P method in 61 cases of NSCLC. Results Expression of PTEN was detected in 30 of 61（49.2%） NSCLC and 14 of 15（93.3%） controls （P〈0.05）; and expression of p53 in NSCLC was higher than that in control group. PTEN expression was associated with histological type, tumor stage and lymph node metastasis p53 expression was associated with lymph node metastasis and gender of patients. The expression of PTEN was negatively corrected with that of p53 （r1=-0. 282,P〈0.05）. Kaplan-Meier survival analysis demonstrated the average survival duration was 36.5 months in PTEN negative group, and 71. 0 months in PTEN positive group; The average survival duration was 70. 1 months in p53 negative group,and 46.0 months in p53 positive group（P〈0.05）. Conclusion Expression status of PTEN and mutated p53 may be used as an indicator for prognosis of NSCLC.