循环抗体与循环抗原组合检测诊断血吸虫病的应用观察

Parallel detection of the circulating antigens and antibodies in the diagnosis of schistosomiasis

目的探讨血吸虫病循环抗原与循环抗体组合检测的诊断效能以及在血吸虫病疫区中的应用价值。方法采用夹心ELISA法和间接ELISA法对不同类型人群的血清标本进行循环抗原和抗SEAIgG抗体检测,比较抗原抗体组合检测的敏感性、特异性,以及在重疫区的检测效果。结果循环抗原与循环抗体平行检测敏感性和特异性分别为97.9%、92.2%;序列检测敏感性和特异性分别为76.0%、99.2%。对血吸虫病疫区粪检阳性人群平行检测阳性率为94.6%(35/37),序列检测阳性率为67.6%(25/37);疫区粪检阴性人群平行检测阳性率为69.8%(97/139),序列检测阳性率为39.6%(55/139)。结论组合检测方案中平行检测在重疫区对有效检出病人具有更高的应用价值。

To determine the diagnostic efficiency of parallel detection of the circulating antigens and antibodies in schistosomiasis, sandwich ELISA and indirect ELISA by using the labeled McAb JPG3 were used to detect the presence of the circulating antigens and the circulating IgG antibodies in serum samples from different kinds of population, and then the sensitivity and specificity of this method of testing as well as the efficiency of the application of this method in heavy endemic area. were determined in comparison with serial test. It was found that the sensitivity and specificity of the parallel test were 97.9 % and 92.2% , however, those of the serial test were 76.00% and 99.2% respectively. The positive rates of parallel test and serial test to detect the stool examination positive for schistosoma eggs in population of the endemic area were 94.6 % （35/37） and 67.6% （25/37）, while those to detect the stool examination-negative for schitosoma eggs were 69.8% （97/139） and 39.6% （55/139） respectively. It is apparent the parallel test for the detection circulating antigens and antibodies in schistosomiasis shows its high diagnostic efficiency, especially in the heavy endemic area of schitosomiasis.