反义c-myc寡脱氧核苷酸对血管平滑肌细胞增殖、迁移和凋亡的影响

Antisense c myc Oligodeoxynucleotides Inhibited Proliferation, Migration of Vascular Smooth Muscle Cells and Induced Apoptosis by Downregulation of c myc Expression

为研究硫代磷酸修饰的反义ｃｍｙｃ寡脱氧核苷酸（ＯＤＮ）对血管平滑肌细胞（ＳＭＣ）的增殖、迁移及凋亡的影响。应用脂质体分别介导硫代磷酸修饰的反义ｃｍｙｃＯＤＮ、正义ｃｍｙｃＯＤＮ和错配ｃｍｙｃＯＤＮ进入体外培养的大鼠主动脉ＳＭＣ内，观察其对ＳＭＣ细胞ｃｍｙｃ表达、增殖、迁移和凋亡的影响。结果：①反义ＯＤＮ降低ｃｍｙｃ蛋白的表达。②反义ｃｍｙｃＯＤＮ抑制ＳＭＣ增殖、迁移，并诱导ＳＭＣ凋亡。而正义ＯＤＮ及错配ＯＤＮ则无上述作用。结果提示：应用反义技术抑制ｃｍｙｃ表达在防治经皮腔内冠状动脉成形术（ＰＴＣＡ）术后再狭窄中具有潜在作用。

To investigate the effects of synthetic phosphorothioate modified antisense oligodeoxynucleotides(ODN), which is complementary to the first five codens of c myc mRNA(AS ODN), on proliferation, migration and apoptosis of vascular smooth muscle cells(SMC), AS ODN were introduced into cultured rat arotic SMCs by lipofectin, observed that antisense ODN inhibited SMC proliferation and migration, and induced SMC apoptosis. These effects were associated with decreased expression of c myc encoded protein which was shown by western blot. ODN with the same nucleotides but a sense sequence or a mismatched sequence did not show same effects as AS ODN did. The results suggested a potential role of antisense strategies designed to inhibit c myc expression for prevention of restenosis following angioplasty.