血管内皮生长因子基因单核苷酸多态性与多囊卵巢综合征易感性研究

Gene single nucleotide polymorphisms of VEGF and the susceptibility for polycystic ovary syndrome

目的:探讨血管内皮生长因子(VEGF)基因的+9812及+13553位点单核苷酸多态性(SNPs)与汉族妇女多囊卵巢综合征(PCOS)的关系。方法:PCOS80例患者为研究组,84例健康妇女为对照组,均为汉族。用基因测序方法测定VEGF基因+9812、+13553位点基因SNPs;比较组间多态基因型分布、等位基因频率及临床代谢参数T、LH、LH/FSH、E2、P、FSH、PRL。计数双侧卵巢基础窦卵泡数(Fc)并进行组间比较。结果:+9812位点多态基因型为CC/CT/CG3种,等位基因为C/T/G,两组CC/CT基因型及等位基因C/T差异无统计学意义(P>0.05),但CG及等位基因G的差异有统计学意义(P<0.05),行相关性分析优势比(OR)为0.302,95%可信区间(CI)为0.102～0.887。+13553位点多态基因型为CC/CT2种,等位基因C/T,两组差异无统计学意义(P>0.05)。PCOS组血清T、LH、LH/FSH、E2、Fc值明显高于对照组,而P明显低于对照组,差异均有统计学意义(P<0.05),FSH、PRL与对照组差异无统计学意义(P>0.05)。结论:汉族妇女VEGF基因+9812位点SNP与PCOS遗传易感性可能有相关性。

Objective： To investigate the frequency of single nueleotide polymorphisms （SNPs） in vascular endothelial growth factor（VEGF） gene at site ＋9812 and ＋13553 in the Han nationality and its role in the pathogenesis of the PCOS. Methods： Eighty cases with PCOS and 84 cases of healthy women as controls were studied. Blood samples were obtained for DNA extraction and sequecing analysis of SNP at ＋1982 and ＋13553 ,follicle count of ovary and levels of sex-hormones were determined. Results：SNP at site ＋9812 CG genotype and G allele had significant difference（ P〈0. 05 ）. There was no significant difference in the frequency of VEGF ＋9812 CC/CT genotype and at site ＋13553 trois （ P〉0.05 ）. The T, LH, LH/FSH, E2, P, CC/CT genotype between PCOS and healthy con- Fc levels of PCOS group were higher than those of the eontrols（P〈0. 05）, but levels of FSH and PRL were not significant different（P〉0.05）. Conclusion：SNP of Han nationality women at ＋9812 site have a high possibility of significant association with the pathogenesis of PCOS.