摘要
目的:探讨卵裂球数量及胚胎碎片对人冻胚卵裂球存活状况的影响。方法:取110例FET(frozen embryo transfer)周期的冻融胚胎,根据卵裂球数量及胚胎碎片分为5组:6~8细胞无碎片组(A组,n=102),4~5细胞无碎片组(B组,n=54),6~8细胞<10%碎片组(C组,n=75),4~5细胞<10%碎片组(D组,n=57),6~8细胞10%~25%碎片组(E组,n=28)。计算各组的胚胎存活率、卵裂球全存活率及卵裂球全死亡率。结果:110个FET周期共解冻胚胎316枚,复苏率68.7%。相同胚胎碎片条件下,6~8细胞与4~5细胞两组间胚胎存活率、卵裂球全存活率、卵裂球全死亡率均无显著差异(P>0.05)。6~8细胞胚胎中,碎片越多,胚胎存活率越低,卵裂球全死亡率越高(P<0.01),而卵裂球全存活率无差异(P>0.05);4~5细胞胚胎中,碎片越多,胚胎存活率(P<0.05)、卵裂球全存活率越低(P<0.01),而卵裂球全死亡率无差异(P>0.05)。结论:胚胎碎片是影响人冷冻胚胎中卵裂球存活状态的重要因素,而卵裂球数量对卵裂球存活无明显影响。
Objective:To explore the impact of blastomere number and fragment on the blastomere survival of human cryopreserved embryos. Methods: A retrospective analysis was performed on l lO frozen embryo transfer (FET)cycles. According to blastomere number and fragment, all thawed embryos were categorized into 5 groups. Group A included 6 - 8 blastomere without fragment( n = 102 ) , group B referred to 4 - 5 blastomere without fragment ( n = 54 ) , group C included 6 - 8 blastomere with 〈 10% of fragment ( n = 75 ), group D referred to 4 - 5 blastomere with 〈10% of fragment(n=57) and group E included 6 -8 blastomere with 10% 25% of fragment ( n = 28 ). The rates of embryos survival, total blastomere survival and total blastomere death were calculated in all groups. Results:A total of 316 embryos were thawed in 110 FET cycles,resulting in embryo survival rate of 68.7%. The rates of embryo survival,total blastomere survival and total blastomere death were similar in comparison group A with group B, and in group C with group D (P〉0. 05 ). With the increase of embryo fragment, there was a significantly lower embryo survival rate, higher total blastomere death(P〈0.01 ) and similar rate of total blastomere survival(P〉0. 05 ) in 6 -8 blastomeres embryos. However, there was a significantly lower rates of embryo survival( P〈0.05 ) and total blastomere survival ( P〈0. 01 ), tomere survival in human cryopreserved embryos.
出处
《现代妇产科进展》
CSCD
北大核心
2009年第11期816-818,共3页
Progress in Obstetrics and Gynecology
关键词
受精
体外
胚胎移植
冻融胚胎
卵裂球数
胚胎碎片
Fertilization in vitro
Embryo transfer
Cryopreserved embryo
Blastomere number
Embryo fragment