卵裂球数量及胚胎碎片对人冻胚卵裂球存活状况的影响

Impact of blastomere number and fragment on the blastomere survival of human cryopreserved embryos

目的:探讨卵裂球数量及胚胎碎片对人冻胚卵裂球存活状况的影响。方法:取110例FET(frozen embryo transfer)周期的冻融胚胎,根据卵裂球数量及胚胎碎片分为5组:6～8细胞无碎片组(A组,n=102),4～5细胞无碎片组(B组,n=54),6～8细胞<10%碎片组(C组,n=75),4～5细胞<10%碎片组(D组,n=57),6～8细胞10%～25%碎片组(E组,n=28)。计算各组的胚胎存活率、卵裂球全存活率及卵裂球全死亡率。结果:110个FET周期共解冻胚胎316枚,复苏率68.7%。相同胚胎碎片条件下,6～8细胞与4～5细胞两组间胚胎存活率、卵裂球全存活率、卵裂球全死亡率均无显著差异(P>0.05)。6～8细胞胚胎中,碎片越多,胚胎存活率越低,卵裂球全死亡率越高(P<0.01),而卵裂球全存活率无差异(P>0.05);4～5细胞胚胎中,碎片越多,胚胎存活率(P<0.05)、卵裂球全存活率越低(P<0.01),而卵裂球全死亡率无差异(P>0.05)。结论:胚胎碎片是影响人冷冻胚胎中卵裂球存活状态的重要因素,而卵裂球数量对卵裂球存活无明显影响。

Objective：To explore the impact of blastomere number and fragment on the blastomere survival of human cryopreserved embryos. Methods： A retrospective analysis was performed on l lO frozen embryo transfer （FET）cycles. According to blastomere number and fragment, all thawed embryos were categorized into 5 groups. Group A included 6 - 8 blastomere without fragment（ n = 102 ） , group B referred to 4 - 5 blastomere without fragment （ n = 54 ） , group C included 6 - 8 blastomere with 〈 10% of fragment （ n = 75 ）, group D referred to 4 - 5 blastomere with 〈10% of fragment（n=57） and group E included 6 -8 blastomere with 10% 25% of fragment （ n = 28 ）. The rates of embryos survival, total blastomere survival and total blastomere death were calculated in all groups. Results：A total of 316 embryos were thawed in 110 FET cycles,resulting in embryo survival rate of 68.7%. The rates of embryo survival,total blastomere survival and total blastomere death were similar in comparison group A with group B, and in group C with group D （P〉0. 05 ）. With the increase of embryo fragment, there was a significantly lower embryo survival rate, higher total blastomere death（P〈0.01 ） and similar rate of total blastomere survival（P〉0. 05 ） in 6 -8 blastomeres embryos. However, there was a significantly lower rates of embryo survival（ P〈0.05 ） and total blastomere survival （ P〈0. 01 ）, tomere survival in human cryopreserved embryos.