“脑舒合剂”对大鼠过度运动后大脑单胺类神经递质的影响

Effects of Naoshu mixture on rats' cerebrum monoamine neurotransmitter after overtraining

目的:观察"脑舒合剂"对大鼠过度运动后大脑单胺类神经递质的影响。为中药制剂消除运动性中枢疲劳提供实验依据。方法:将雄性SD大鼠40只(体重230±20克),随机分为:空白对照组(C组)、负重游泳组(O组)、负重游泳加低剂量组(低药组n=10,生药10g/kg)和负重游泳加高剂量组(高药组n=10,生药30g/kg)。每天C组和O组灌等量蒸馏水,低药组和高药组灌服"脑舒合剂"。除C组外,其余三个组递增负荷游泳至第八周。结果:经过8周大负荷游泳后,O组大鼠大脑皮质的去甲肾上腺素(NE)阳性表达水平显著性降低,低药组和高药组无显著性变化;O组大鼠大脑皮质的五羟色胺(5-HT)阳性表达水平显著性升高,低药组和高药组无显著性变化。结论:"脑舒合剂"有助于维持大脑皮质的NE水平,抑制5-HT水平升高,使大脑皮质的兴奋与抑制过程处于相对平衡状态,对过度运动后的大脑功能有良性作用。

Objective： This research was designed to observe the effects of Naoshu mixture on rats＇ cerebral monoamine neurotransmitter after they had overtraining.Methods： 40 male SD rats（weight 230±20g） were divided randomly into 4 groups： control group（group C,n=10）;burdened swimming group（group O,n=10）;burdened swimming and low dose group（low dose group,n=10,crude drug 10g/kg）;burdened swimming and high dose group（high dose group,n=10,crude drug 30g/kg）.Equivalent distilled water was given to group C and group O everyday.All the groups were given increase load swimming for eight weeks,except group C.Results： The protein expression of NE in rats＇ cerebral cortex was reduced significantly after overtraining in group O（p0.05）.But the expression of NE had no significant changes in Naoshu mixture groups.The expression of 5-H in rats＇ cerebral cortex had significant increase in group O（p0.05）,but there was no significant change in low dose and high dose group.Conclusion： Naoshu mixture could raise the expression of NE in cerebral cortex,keep the exciting and the depressing of cerebral cortex relative balance,and maintain better function of cerebral cortex after overtraining.