风信子DFR基因全长cDNA的克隆及序列分析

Cloning and Sequencing Full-length cDNA of DFR Gene in Hyacinthus

以蓝色风信子完全露出蓝色的花蕾为材料,利用RT-PCR和RACE技术,获得风信子DFR基因的全长cDNA。该cDNA全长1252bp,开放阅读框为1098bp,编码366个氨基酸。Blast搜索结果显示,风信子DFR基因核苷酸序列与其它植物已报道的DFR基因具有66%～77%的相似性,氨基酸序列有62%～73%的相似性。聚类分析表明,最先与风信子聚类合并的是鸢尾,其次与其它单子叶植物聚类,最后与双子叶植物聚类。

A total length DFR gene was cloned with RT-PCR and RACE technology from flower bud with blue petals of Hyacinthus orientalis L.. Hyacinthus DFR cDNA was 1252 bp in length. Within the full-length cDNA, a clear open reading frame （ORF） was 1098 nucleotides, coding 366 amino acids. The results of homologous analysis in GenBank demonstrated that the sequence had 66 %-77 % identity on the nucleotide sequence and 62 %-73% identity on the deduced amino acid sequence. The results of phylogenetie analysis showed that DFR from Hyacinthus orientalis and from Iris x hollandica clustered together firstly, and then came those from other monocotyledon, dicotyledon came subsequently.