植物组织双向电泳样品制备方法研究进展

Research Progress on Protein Extraction from Plant Tissues for Two-dimensional Gel Electrophoresis

双向电泳是目前应用最广泛的蛋白分离技术,该技术的核心是蛋白样品的制备。植物组织中通常含有大量蛋白酶和次生代谢物,这严重干扰蛋白提取、分离和鉴定。从植物组织中提取高质量的蛋白样品用于蛋白组分析充满挑战,简要阐述了植物组织蛋白提取的关键点,特别是植物组织中的次生代谢物及去除方案。此外,还对三种植物蛋白提取方法、三种方法的优缺点及适用植物组织作了概述。

The two-dimensional gel electrophoresis （2-DE）is the most popular techniques for protein separation, and the key technology of 2-DE is the protein extraction. Plant tissues usually contain a large number of proteases and secondary metabolites, and these substances severely interfere with protein extraction, separation, and identification. Due to the reason mentioned above, preparing the high-quality protein samples from plant tissues for proteomic analysis is always filled with lots of challenges. In this paper, the author briefly describes the three critical points on protein separation, especially secondary metabolites in plant tissues and the strategies of getting rid of secondary metabolites. In addition, the author also provides updated overviews of three total protein extraction methods, their advantages and disadvantages and their applications for protein extraction from plant tissues.