芒(Miscanthus sinensis Anderss)花粉生活力测定方法比较

Comparison of Pollen Viability Determining Methods for Miscanthus sinensis Anderss

采用离体萌发法、FDA染色法和I2-KI染色法测定芒离体花粉的生活力,并对测定效果进行比较。结果表明,利用离体萌发法测得芒离体花粉的平均初始萌发率为82.6%,并且花粉萌发和生长很快,5min即开始萌发,培养30min后花粉管平均长度达到了145.77μm,但花粉的平均萌发率下降很快,室温保存90min的花粉其萌发率已下降至3.0%。利用FDA染色法和I2-KI染色法测定的芒花粉初始生活力与离体萌发法的结果基本一致,分别为84.6%和86.6%,但这两种染色法在跟踪测定芒离体花粉的生活力时易误判,不适合用于跟踪测定花粉生活力的变化。利用离体萌发法能够准确有效地测定芒花粉生活力的变化规律,而FDA染色法和I2-KI染色法适合用于测定芒花粉的初始生活力。

The pollen viability of Miscanthus sinensis was determined by in vitro pollen germination method, I2-KI staining method and FDA staining method, respectively. These three methods were analyzed to find their advantages, disadvantages and effectiveness. The results showed that with the in vitro pollen germination method, the pollens germinated in 5 minutes and the pollen tubes reached an average length of 145.77 μm in 30 minutes. The average initial pollen germinating rate was 82.6%, but it was decreased sharply to 3.0% when the pollens were stored for 90 minutes at room temperature. The initial staining rate by FDA method and I2-KI method was 84.6% and 86.6% respectively, close to that of in vitro pollen germination method. However, the last two methods could not accurately distinguish the inactive pollens from the pollens having been stored for a longer time. In vitro pollen germination method could be an accurate and effective method to test the change of the pollen viability. I2-KI staining method and FDA staining method could be used to test the initial pollen viability effectively.