McAb共激活T细胞介导肝癌细胞前后的膜分子与TCRVβ基因的表达

MEMBRANE MOLECULES AND TCR Vβ GENES EXPRESSION IN McAbCOSTIMULATED T CELLS BEFORE AND AFTER INCUBATED WITH HEPATOCELLULAR CARCINOMA CELL LINE

Ｔ淋巴细胞活化是一个涉及多种膜表面分子和受体以及一系列相关多肽的复杂过程，为了使Ｔ细胞发挥更好的识别和杀伤癌细胞的功能，采用抗ＣＤ３、ＣＤ２８、ＣＤ８０（Ｂ７１）、ＣＤ２、ＣＤ５８ＭｃＡｂ分别刺激健康人ＰＢＬｓ后作用肝癌细胞，对作用前后ＰＢＬｓ用ＦＡＣＳ进行表型分析，结果发现：作用后ＣＤ３和ＣＤ８分子表达比作用前明显增高，而ＣＤ４分子无显著变化，同时基因家族采用ＲＴＰＣＲＳｏｕｔｈｅｒｎ印迹分析ＴＣＲＶβ基因１～２０亚家族表达水平与特征，健康人ＰＢＬｓ分别加入ＩＬ２、ＰＨＡ、抗ＣＤ３和ＣＤ３＋ＣＤ２８、ＣＤ２８＋ＣＤ８０、ＣＤ２＋ＣＤ５８作用肝癌细胞（ＢＥＬ７４０２）前表达水平平均约为５％，作用ＢＥＬ７４０２后表达水平约为１３％～２５％，其特征为Ｖβ７增高，这提示在癌抗原的参与下ＭｃＡｂ共刺激的Ｔ细胞活化，ＴＣＲ接受ＡＰＣ相应抗原的刺激，具有该ＴＣＲ的淋巴细胞迅速增殖而成为针对抗原的Ｔ细胞克隆，发挥其识别和杀伤癌细胞的作用，这将为肿瘤生物治疗的研究提供分子免疫学依据。

To make T lymphocyte recognizing and killing the target tumor cell efficiently,FACS analysis was used to compare the membrane molecules expression in antiCD3,CD28,CD80,CD2,CD58 McAbcostimulated PBLs before and after incubated with hepatocellular carcinoma cell line(BEL7402),RTPCR was done to analyse TCR Vβ gene 1～20 subfamily expression.After incubated with BEL7402 cell line,the levels of TCR Vβ expression increased from 5% to 13%～25%,mainly Vβ7.CD3 and CD8 molecule expression were also significantly increased but no changes in CD4 molecule.At the presence of tumor antigens,the McAbcostimulated T cells were activated,TCR was stimulated by antigens presented by APC.T cells bearing this type of TCR may proliferate rapidly to antigenspecific clones,then recognize and kill the rumor cells.This model could provide a molecular basis for tumor biotherapy.