摘要
T淋巴细胞活化是一个涉及多种膜表面分子和受体以及一系列相关多肽的复杂过程,为了使T细胞发挥更好的识别和杀伤癌细胞的功能,采用抗CD3、CD28、CD80(B71)、CD2、CD58McAb分别刺激健康人PBLs后作用肝癌细胞,对作用前后PBLs用FACS进行表型分析,结果发现:作用后CD3和CD8分子表达比作用前明显增高,而CD4分子无显著变化,同时基因家族采用RTPCRSouthern印迹分析TCRVβ基因1~20亚家族表达水平与特征,健康人PBLs分别加入IL2、PHA、抗CD3和CD3+CD28、CD28+CD80、CD2+CD58作用肝癌细胞(BEL7402)前表达水平平均约为5%,作用BEL7402后表达水平约为13%~25%,其特征为Vβ7增高,这提示在癌抗原的参与下McAb共刺激的T细胞活化,TCR接受APC相应抗原的刺激,具有该TCR的淋巴细胞迅速增殖而成为针对抗原的T细胞克隆,发挥其识别和杀伤癌细胞的作用,这将为肿瘤生物治疗的研究提供分子免疫学依据。
To make T lymphocyte recognizing and killing the target tumor cell efficiently,FACS analysis was used to compare the membrane molecules expression in antiCD3,CD28,CD80,CD2,CD58 McAbcostimulated PBLs before and after incubated with hepatocellular carcinoma cell line(BEL7402),RTPCR was done to analyse TCR Vβ gene 1~20 subfamily expression.After incubated with BEL7402 cell line,the levels of TCR Vβ expression increased from 5% to 13%~25%,mainly Vβ7.CD3 and CD8 molecule expression were also significantly increased but no changes in CD4 molecule.At the presence of tumor antigens,the McAbcostimulated T cells were activated,TCR was stimulated by antigens presented by APC.T cells bearing this type of TCR may proliferate rapidly to antigenspecific clones,then recognize and kill the rumor cells.This model could provide a molecular basis for tumor biotherapy.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第5期334-337,共4页
Chinese Journal of Immunology
基金
国家自然科学基金
广东省五个一工程粤卫科资助
关键词
肝肿瘤
肝癌细胞
T细胞介导
膜分子
McAb
McAbcostimulation
Hepatocellular carcinoma
Membrane molecule
TCR Vβ gene