IL-12、IL-7、IL-2体外协同诱导人PBMC增殖和PBMC杀瘤活性的实验研究

SYNERGISTIC INDUCEMENT OF PROLIFERATION AND ANTITUMOR ACTIVITY OF HUMAN PBMC BY IL-12,IL-7 AND IL-2 IN VITRO

研究了重组ＩＬ１２、ＩＬ７、ＩＬ２协同对健康人外周血单个核细胞（ＰＢＭＣ）的体外增殖和杀瘤活性的影响。结果表明，高浓度ＩＬ２与低浓度ＩＬ２协同可引起ＰＢＭＣ明显增殖，若在该培养体系中加入低浓度的ＩＬ２，可使这种增殖效应显著增强。如果加大ＩＬ１２剂量时会产生更强的细胞毒活性，并呈现剂量依赖关系。在此培养条件下的培养上清中亦检出了较高水平的ＩＦＮγ。对培养３～１４ｄ的协同刺激细胞表型分析显示，ＣＤ５６抗原阳性细胞百分率呈递增趋势，提示ＣＤ５６＋的ＮＫ细胞构成了产生增殖和杀伤效应的主要细胞群体。

The synergistic effects of recombinant IL12、IL7 and IL2 on the proliferation of human normal peripheral blood mononuclear cell(PBMC) and their antitumor effect had been studied.Results showed that high dose of IL7 plus low dose of IL12 could obviously promote PBMC proliferation.The promotion could be dramatically enhanced by addition of low dose of IL2.If raised the concentration of IL12,the cytotoxic activity could also raised,it suggested that this was a dosedependent effect.High concentrations of IFNγ could be detected in the culture supernatant.According to the phenotype analysis of the synergistic treated PBMC during 3 to 14 days,the percentage of CD56 positive cells raised gradually among the total PBMC.It suggested that CD56+ NK cell was the main proliferation subset which played the cytotoxic effect.