摘要
目前,在水稻中已克隆出GA受体GID1基因,其功能丧失会导致植株矮化。同时,在拟南芥中也克隆出3个相关的GA受体基因。根据它们氨基酸序列的保守区设计简并引物,在不同作物中进行扩增。扩增产物进行回收及测序,根据这些片段的序列从数据库中得到对应的EST序列和氨基酸序列。结果表明:水稻与高粱、小麦、玉米(GID1A和GID1B)和棉花中GA受体的氨基酸序列具有较高的同源性,分别为81.44%、81.36%、80.50%、79.14%和63.13%。
GID1 as gibberellin acid （ GA） acceptor has been cloned in rice. Loss of its function can cause dwarfing of plant height, meanwhile, three GA acceptors have been cloned in Arabidopsis. According to the conserved domains, a pair of degenerate primer was designed. Amplifications were carried out among several different crops with those pairs of degenerate primers. Subsequently, these ：fragments were purified and sequenced, and these sequences were compared with a software to find their corresponding expressed sequence tag （EST）. According to those EST sequences, amino acid sequences coding these genes were deduced in sorghum, wheat, maize（GID1A and GID1B）and cotton. Their amino acid sequences had higher similarity to rice with 81.44%, 81.36%, 80. 50%, 79. 14%, 63.13% , respectively.
出处
《青岛农业大学学报（自然科学版）》
2009年第4期309-312,共4页
Journal of Qingdao Agricultural University(Natural Science)
基金
青岛农业大学博士启动基金(630716)
关键词
GA受体
同源基因
进化分析
gibberellin acid （ GA ） acceptor
homologous gene
evolutionary analysis
