北京HIV抗体阳性MSM中HLA-A-B-DRB1基因多态性与病毒载量相关性的初步研究

Polymorphisms of HLA-A,-B,and-DRB1 loci and its correlation with viral loads among HIV-positive MSM in Beijing

目的从基因水平了解北京艾滋病病毒(HIV)抗体阳性的男男性行为者人群(MSM)中,人类白细胞抗原(HLA)-A、-B、-DRB1位点的等位基因频率,分析其与HIV感染者自身病毒载量的关系。方法应用聚合酶链反应-序列特异性引物技术(PCR-SSP),对北京32例HIV阳性MSM进行了HLA-A、-B、-DRB1等位基因分型。结果鉴定了12个HLA-A等位基因,21个HLA-B等位基因,12个HLA-DRB1等位基因。最常见的等位基因分别为A*02(26.56%)、B*40(17.19%)和DRB1*15(20.31%)。含有A*02等位基因组者的血浆病毒载量,较不含有此等位基因组者低(P=0.002),而含有HLA-B*40和HLA-DRB1*15等位基因组者的血浆病毒载量,较不含有对应等位基因组者高(HLA-B*40:P=0.799;HLA-DRB1*15:P=0.021)。结论北京HIV阳性MSM人群HLA-A、-B、-DRB1基因多态性较高。HLA-A*02等位基因在该人群中可能与延缓AIDS疾病进程相关,而HLA-DRB1*15等位基因可能与加速该人群AIDS疾病进程相关。

Objective To explore allele frequency of HLA A, HLA-B, HI.A DRB1 among HIV positive men who have sex with men （HIV＋MSM） in Beijing and to analyze its correlation with viral load in plasma. Methods Polymerase chain reaction-sequence specific primers （PCR SSP） were used to determine HI.A-A, HLA-B and HLA- DRB1 alleles among 32 HIV＋MSM in Beijing. Result 12 A, 21 B and 12 DRB1 alleles were detected. The most common HLA-A, HLA B and HLA DRB1 allele was A ＊ 02, B ＊ 40, DRB1＊ 15 with the frequency of 26. 56%, 17.19% and 20.31 %, respectively. The plasma viral load of individuals with HLA A ＊ 02 alleles was lower than in those without HLA-A ＊ 02 alleles （P=0. 002）, however, the plasma viral load in individuals with either HLA-B 40 or HLA-DRB1 ＊ 15 was higher than in those without HLA-B ＊ 40 or HLA DRB1 ＊ 15, （HLA-B ＊ 40, P= 0. 799, HLA-DRB1 ＊ 15, P=0. 021）. Conclusion The polymorphisms of HLA A, -B, and -DRB1 loci are high, HLA-A ＊ 02 allele＊ may be associated with slower HIV disease progression, whereas HLA-DRB1 ＊ 15 alleles are potentially associated with faster disease progression among HIV-positive MSM in Beijing.