摘要
目的:建立RP—HPLC同时测定地榆提取物中没食子酸和鞣花酸含量的测定方法。方法:色谱柱为Shimadzu ODS C_(18)柱(4.6mm×150mm,5μm),流动相:甲醇-0.1%磷酸溶液梯度洗脱。检测波长280 nm,流速1.0 mL·min^(-1),柱温为室温。结果:没食子酸和鞣花酸在0.2～1μg范围内线性关系良好,其平均回收率分别为98.7%和99.4%,RSD分别为1.75%和2.11%。结论:本方法操作简便,结果准确可靠,适于地榆中没食子酸和鞣花酸的同时测定。
An Rp-HPLC method for the determination of gallic acid（GA） and ellagic acid（EA） in Sanguisorba officinalis L.is reported.Separation was achieved on a Shimadzu ODS C18 column（4.6 mm×150 mm,5μm） with methanol-0.1%phosphoric acid gradient elution.The detection wavelength was 280 nm and the flow rate was 1.0 mL·min^-1.It was found that GA and EA have good linear relationship in the range of 0.2～1μg.The average recoveries of GA and EA was 98.7%and 99.4%respectively.The proposed method is simple,rapid and accurate.It could be used to determine GA and EA in Sanguisorba officinalis L.simultaneously.
出处
《现代科学仪器》
2009年第5期69-70,共2页
Modern Scientific Instruments
关键词
地榆
没食子酸
鞣花酸
HPLC
Sanguisorba officinalis L.
Gallic acid
Ellagic acid
HPLC
