亚砷酸钠致SV-HUC-1细胞氧化应激作用的研究

Oxidative Stress Induced by NaAsO_2 in SV-HUC-1 Cell

目的探讨亚砷酸钠(NaAsO2)对人膀胱上皮永生化细胞SV-HUC-1的氧化应激作用。方法以不同浓度NaAsO(20、0.1、0.2、0.5、1、2、4、6、8、10、20μmol/L)对SV-HUC-1细胞进行染毒,采用四甲基偶氮唑(MTT)比色法检测细胞活力,利用2’,7’-二乙酰二氯荧光素(DCFH-DA)检测细胞内活性氧(ROS)水平,分别应用DTNB比色法、硫代巴比妥酸比色法和黄嘌呤氧化法检测细胞内谷胱甘肽(GSH)含量、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活力。结果与空白对照组比较,全部染毒组细胞活力均下降(P<0.05);染毒24h后,全部染毒组ROS水平均显著升高(P<0.05);2、4μmol/LNaAsO2组细胞GSH含量显著升高(P<0.05);全部染毒组细胞MDA含量均无变化(P>0.05);全部染毒组细胞SOD活力均显著降低(P<0.05)。结论氧化应激可能是NaAsO2致SV-HUC-1细胞毒性作用的机制之一。

Objective To study the state of oxidative stress induced by sodium arsenite （NaAsO2） in SV-HUC-1 cell. Methods MTT assay was used to evaluate the viability of the cells. The level of ROS was detected by staining cells with DCFH-DA. The content of GSH and MDA were measured by DTNB and thiobarbituric acid methods. The activity of SOD was measured by xanthine oxidase method. Results Compared with the control group, the viability cells decreased in all the treated groups （P〈0.05）, after 24 h treatment, the level of ROS in all the treated groups were significantly elevated （P〈0.05）, the content of GSH increased significantly in the cells treated at dose of 2 and 4 μmol/L NaAsO2 （P〈0.05）.For all the treated groups, there was no significance of difference for the content of MDA （P〉0.05）, the activity of SOD in all the treated groups was significantly decreased （P〈0.05）. Conclusion Oxidative stress may be an important mechanism for the toxicity of sodium arsenite to SV-HUC- 1 cell.