摘要
目的:建立测定人体血浆中喷昔洛韦高效液相色谱法,并测定健康志愿者口服泛昔洛韦片12h内血药浓度。方法:采用6%高氯酸直接沉淀提取血浆,色谱柱为C18(250mm×4.6mm,5μm),0.4%磷酸-甲醇(95∶5)为流动相,流速0.8mL·min-1,荧光检测波长为λex=270nm,λem=375nm。结果:喷昔洛韦本法线性范围为0.05~5μg·mL-1(r=0.9999,n=7);最低定量限0.05μg·mL-1;低、中、高3种浓度的平均回收率分别为(97.80±2.28)%,(96.60±0.37)%,(96.58±0.38)%;日内、日间精密度RSD均<4%。结论:本检测方法简便、快速、经济、准确,可用于测定人血浆中喷昔洛韦的浓度。
Objective:To establish an HPLC method for the determination of penciclovir in human plasma,and to determine the concentration of penciclovir in human plasma after oral of famciclovir tablets into healthy volunteers within 12 hours.Methods:The penciclovir was extracted from plasma by 6% perchloric acid.Chromatographic separation has been achieved on C18 column(250 mm×4.6 mm,5 μm) with 0.4% phosphoric acid-methanol(95∶5)as the mobile phase at a flow rate of 0.8 mL·min-1;The fluorescence detection at λex=270 nm and λem=375 nm.Results:The linear range was 0.05-5 μg·mL-1(r=0.9999,n=7).The minimum ration limit was 0.05 μg·mL-1.The average recoveries of low,middle and high concentration were (97.80±2.28)%,(96.60±0.37)% and (96.58±0.38)%,RSDs of inter and intra-day precisions were less than 4%.Conclution:The method is simple,rapid,economic and accurate for determination of penciclovir in human plasma.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第12期2007-2009,共3页
Chinese Journal of Pharmaceutical Analysis