RP-HPLC法测定柳叶鬼针草地上部分中3种黄酮苷的含量

RP-HPLC determination of three flavonoids in aerial parts of Bidens ceruna L.

目的:对柳叶鬼针草地上部分中的3种主要黄酮苷类成分木犀草素-7-O-β-D-吡喃葡萄糖苷(化合物Ⅰ)、槲皮苷(化合物Ⅱ)、紫铆因-4-甲氧基-4'-O-β-D-吡喃葡萄糖苷(化合物Ⅲ)进行含量测定。方法:采用反相高效液相色谱法,使用ZORBAX SB-C18(250mm×4.6mm,5μm)色谱柱,以乙腈-0.1%醋酸水溶液为流动相进行梯度洗脱(0～30min,16∶84;30～46min,24∶76),流速1.0mL·min-1,检测波长350nm,柱温25℃。结果:化合物Ⅰ～Ⅲ进样量在0.01～5.00μg范围内均有良好的线性关系(r≥0.9992,n=8)。化合物Ⅰ～Ⅲ的平均回收率(n=6)分别为100.3%(RSD为2.0%)、100.4%(RSD为1.8%)和102.6%(RSD为1.9%)。结论:本方法能够准确测定柳叶鬼针草中3种主要黄酮苷类成分含量。

Objective：To determine three flavonoids,luteolin-7-O-β-D-glucopyranoside （compoundⅠ）,quercitrin （compound Ⅱ）,butein-4-methoxyl-4＇-O-β-D-glucopyranoside （compound Ⅲ） in aerial parts of Bidens ceruna.Methods：Separation was carried out on a ZORBAX SB-C18 column （250 mm×4.6 mm,5 μm）.The mobile phase was acetonitrile-water（containing 0.1% acetic acid） with gradient elution （0-30 min,16∶84;30-46 min,24∶76）.The flow rate was 1.0 mL·min-1 at 25 ℃,and the detection wavelength was set at 350 nm.Results：The linearity was in the range of 0.01-5.00 μg for compoundsⅠ-Ⅲ （r≥0.9992,n=8）;The average recoveries （n=6） for compound Ⅰ-Ⅲ were 100.3%（RSD=2.0%）,100.4%（RSD=1.8%） and 102.6%（RSD=1.9%） respectively.Conclusion：This method is accurate,reliable and sensitive for determination of three flavonoids in Bidens ceruna L.