HPLC法同时测定高良姜中3种黄酮类成分的含量

HPLC simultaneous determination of 3 flavonoids in Alpinia officinarum Hance

目的:建立同时测定高良姜药材中高良姜素、山奈素-4′-甲醚、高良姜素-3-甲醚含量的高效液相色谱法。方法:采用Promosil C18(4.6mm×250mm,5μm)色谱柱,以乙腈(A)-0.05%磷酸水溶液(B)为流动相,梯度洗脱(0～30min,34%A→38%A;30～55min,38%A),流速1.0mL·min-1,检测波长264nm,柱温30℃,进样量10μL。结果:高良姜素、山奈素-4′-甲醚、高良姜素-3-甲醚的线性范围分别为12.18～121.8μg·mL-1(r=0.9999),4.44～44.4μg·mL-1(r=0.9999),2.53～25.32μg·mL-1(r=0.9998);平均加样回收率(n=9)分别为99.2%,100.5%,100.4%。结论:本法准确、简便,具有良好的重复性,可为高良姜药材质量控制提供依据。

Objective：To develop an HPLC method for simultaneous determination of galangin,kaempferide-4′-methylether and galangin-3-methylether in the hebal of Alpinia officinarum Hance from different places.Methods：The separation was performed on a Promosil C18 （4.6 mm×250 mm,5 μm） column by gradient elution with acetonitrile（A）-0.05% phosphoric acid（B） （0-30 min,34% A→38% A;30-55 min,38% A） as the mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 264 nm,and the column temperature was 30 ℃.Results：The calibration curves of galangin,kaempferide-4′-methylether and galangin-3-methylether were linear in the ranges of 12.18-121.8 μg·mL-1（r=0.9999）,4.44-44.4 μg·mL-1（r=0.9999） and 2.53-25.32 μg·mL-1（r=0.9998） respectively;The mean recoveries（n=9） of them were 99.2%,100.5% and 100.4% respectively.Conclusion：The developed method is simple,accurate,reliable,and is helpful to control the quality of Alpinia officinarum Hance.