苍南四季柚果皮挥发油成分的气相色谱-质谱联用分析

GC-MS analysis of chemical constituents of volatile oil from peels of Cangnan Sijiyou

目的:分析苍南四季柚果皮挥发油成分。方法:利用水蒸气蒸馏法提取四季柚果皮挥发油,用气质联用(GC-MS)法对挥发油成分进行分析,色谱柱为HP-5MS毛细管柱(30m×0.25mm×0.25μm),结合计算机检索技术对分离的化合物进行鉴定,应用色谱峰面积归一化法计算各成分的相对百分含量。结果:共分离出58个组分,经NIST谱库检索,鉴定了其中52个化合物。分离出四季柚果皮挥发油主要成分有:D-柠檬烯47.51%、β-月桂烯(18.63%)、努特卡酮(14.66%)、喇叭茶萜醇(2.09%)、β-蒎烯(2.03%)。结论:对四季柚果皮挥发性成分的研究,为进一步开发利用提供了依据。

Objective：To analyze the chemical constituents of volatile oil from peels of Cangnan Sijiyou.Method：The chemical compositions of the volatile oil obtained by steam distillation were analyzed by GC-MS equipped with an HP-5MS capillary column（30 m×0.25 mm×0.25 μm）.The constituents were identified by their mass spectra.Their relative percentages were calculated with peak area normalization method.Results：Fifty-eight chemical constituents in peels of Sijiyou were separated;of which fifty-two compounds of the oil were characterized by MS with NIST.The major chemical constituents are D-limonene （47.51%）,β-myrcene （18.63%）,nootkatone （14.66%）,ledol （2.09%）,β-pinene （2.03%）.Conclusion：The determination of chemical constituents of volatile oil from peels of Sijiyou will provide important foundation for further exploitation of Cangnan Sijiyou.