实时荧光PCR芯片及其检测平台的设计

Design on Real-time PCR Chip and Detecting Platform

研制了一款实时荧光PCR芯片及其检测平台。其中PCR芯片实现样品的预处理及实时荧光反应等功能;检测平台实现实时荧光检测、分析及处理等功能。该检测平台由注射泵系统、实时荧光信号采集系统和PCR温度控制系统等组成。并成功地实现了从血液中提取白细胞及同一腔体中PCR反应、检测以及分析等。实验结果表明,该系统能满足实时荧光PCR芯片的控制与检测实验结果,达到了设计各项指标。

This paper introduced a section of real-time fluorescent polymerase chain reaction（PCR） chip and its detecting platform development.It integrated the biological sample＇s pretreatment and the real-time fluorescent PCR reaction to one chip.It could separate the white blood cell from the blood and then carry out the PCR reaction in the identical chamber.The detecting platform was composed of syringe pump module,temperature control module and fluorescence sampling module,which could meet the needs for real-time PCR chip control and detection requirements.