甲基叔丁基醚遗传毒性研究

Study on the Genotoxicity of Methyl Tertiary Butyl Ether

甲基叔丁基醚（ＭＴＢＥ）是一种新型的汽油添加剂，被用来提高汽油辛烷值和减少汽车尾气中有害物质的排放。我们采用Ａｍｅｓ试验、程序外ＤＮＡ合成（ＵＤＳ）试验和细胞微核试验等反映不同遗传学终点的系列试验，检测了国产ＭＴＢＥ的遗传毒性。在Ａｍｅｓ试验（ＴＡ９８和ＴＡ１００菌株）中，在加和不加Ｓ－９的情况下，ＭＴＢＥ均未表现出致突变性。大鼠肝细胞ＵＤＳ试验显示，ＭＴＢＥ具有轻微的ＤＮＡ损伤作用。在ＮＩＨ３Ｔ３细胞微核试验中，ＭＴＢＥ呈现阴性结果。上述结果提示。

Methyl tertiary butyl ether(MTBE),a new gasoline additive,is added to gasoline as an octane enhancer and used to reduce automotive emissions.Using Ames test,unsheduled DNA synthesis(UDS) assay and cell micronucleus test,the authors evaluated the genotoxicity of domestic produced MTBE.MTBE did not show any mutagenic potential in Ames assay(TA98,TA100 strains)with or without rat liver derived metabolic activation system (S 9).Rat primary hepatocyte UDS assay showed that MTBE could damage DNA.In NIH 3T 3 cell micronucleus test,MTBE showed negative results.It could be concluded that MTBE has some genotoxicity at DNA level.