深圳市吸毒人群和异性性传播HIV-1的分子流行病学研究

A molecular epidemiological study of HIV-1 transmission among injection drug users and heterosexuals in Shenzhen City

目的了解深圳市吸毒人群和异性性传播感染者HIV-1的分子流行病学情况。方法应用BigDye链终止反应试剂盒,对2000-2008年收集的62例深圳市吸毒人群和61例异性性传播感染者HIV-1RNA2.6-kbgag-RT区的核苷酸序列进行测定,并确定其基因型分布。结果样品中共存在CRF07BC,CRF08BC,CRF01AE三种重组株和B,B′,C三种亚型。在吸毒人群HIV-1中,CRF07BC和CRF08BC是主要基因型,在样品中的比例为37.1%(23/62)和45.2%(28/62),CRF01AE、B′、B、C亚型所占比例分别为9.7%(6/62)、4.8%(3/62)、1.6%(1/62)和1.6%(1/62)。同样,异性性传播HIV-1感染者的基因型也以CRF07BC和CRF08BC为主,其比例为29.5%(18/61)和41.0%(25/61),而CRF01AE、B′、B亚型分别为21.3%(13/61)、6.6%(4/61)、3.3%(2/61),无C亚型存在。结论深圳市异性性传播HIV-1感染者中流行的病毒基因型主要来自于当地吸毒人群。

Objective To investigate the molecular epidemiology of HIV-1 among injection drug users （IDUs） and het- erosexuals in Shenzhen City. Methods HIV-1 positive plasma samples were obtained from 62 injection drug users and 61 heterosexuals in Shenzhen from 2000 to 2008. The distribution of HIV - 1 genotypes was determined based on nucleotide sequences of 2. 6-kb gag-RT regions on both strands using BigDye terminator reaction kits. Results There were 6 HIV-1 strains including 3 circulating recombinant forms （CRFs） of CRF07 BC, CRF08 BC, CRF01 AE and 3 subtypes of B＇, B, C found in all samples. Among IDUs, CRF07 BC and CRF08 BC were the dominant subtypes, with the percentages of 37. 1% （23/62） and 45.2% （28/ 62） , respectively. The percentages for CRF01 AE, subtype B＇, B, C were 9. 7% （6/62） , 4. 8% （3/62） , 1.6% （ 1/62）, and 1.6% （ 1/62）, respectively. Similarly, a majority of heterosexuals were infected with CRF07 BC （ 18/61, 29.5% ） and CRF08 BC （25/61, 41.0% ）. The percentages for CRF01 AE, subtype B＇and B were 21.3% （ 13/61 ）, 6.6% （4/61） and 3.3% （2/61）, respectively. No subtype C was found among heterosexuals. Conclusion The heterosexual HIV-1 epidemic in Shenzhen appears to derive from the local IDU epidemic.