摘要
目的探讨急性热应激（HS）和热性惊厥（FC）对大脑皮层胆囊收缩素（CCK）和催乳素（PRL）定位表达的影响。方法采用热水浴诱导21日龄大鼠FC模型，应用免疫组织化学技术，对HS和FC大鼠CCK和PRL在大脑皮层的定位表达进行比较分析。结果（1）HS组CCK和PRL阳性细胞在大脑皮层分布极为相似，免疫染色有共深或共浅的倾向。（2）HS组和FC组大脑皮层CCK、PRL阳性细胞数明显高于对照组（P〈0．01）。FC组大鼠大脑皮层各区CCK阳性细胞数明显高于HS组（P〈0．01）。FC组大鼠大脑皮层杏仁周皮质、内嗅皮质、压部后皮质PRL阳性细胞数与HS组差异无显著性（P〉0．05），嗅周皮质和顶皮质PRL阳性细胞数明显高于HS组，差异有非常显著性（P〈0．01）。（3）HS组大脑皮层CCK和PRL阳性细胞阳性单位呈明显正相关（Y=7．939＋1．36X，r=0．97，P〈0．01），而FC组两者无相关性（r=0．47，P〉0．05）。结论（1）CCK参与海马等前脑结构对惊厥活动的中枢调控机制。（2）PRL与CCK协同作用参与对HS的中枢调控。
Objective To analyze the distribution of cholecystokinin （CCK） and prolactin （PRL） positive cells in rat＇ s brain following heat stress （HS） and febrile convulsion （FC）. Methods Acute heat stress model of seizure induced by warm water was developed in this study. Adjacent section immunohistochemical staining method was used to observe expression of CCK and PRL in cerebral cortex. Results （ 1 ） There were similar distributions of CCK and PRL positive cells in cerebral cortex of HS group. （2） Both HS and FC rats showed more positive neurons in cerebral cortex than those in control group（P 〈 0.01 ）. There were significant more CCK positive neurons in cerebral cortex than that in HS group（ P 〈 0. 01 ）, however, no significant difference of PRL positive neurons was found in piriform cortex and entorhinal cortex between HS and FC group（P 〉0. 05） ,but the difference was significant in perirhinal cortex and parietal cortex. （3）Correlation and regression analysis of the data of CCK and PRL positive units demonstrated that the immunoreacrive intensity of CCK and PRL had a positive linear correlation in cerebral cortex of HS group（ Y =7.939 ＋ 1.36X,r=0.97,P 〈0.01）,but no correlation was found in cerebral cortex of FC group（r =0.47,P 〉 0. 05）. Conclusion （ 1 ） CCK may involve in anti-convulsant mechanisms in response to FC. （2） There may be a synergistic action of PRL and CCK in the central control of HS.
出处
《中国小儿急救医学》
CAS
2009年第6期561-563,共3页
Chinese Pediatric Emergency Medicine
