吉林省普通大豆品种(系)异黄酮含量分析

Analyse of Isoflavones Content in Normal Soybean Varieties(Lines)in Jilin Province

利用超声波法对吉林省26份普通大豆品种（系）大豆异黄酬含量进行HPLC测定。建立了一种HPLC测定大豆籽粒中异黄酮含量的方法。结果表明：异黄酮各异构体在50～1000μmol·L^-1范围内线性系数良好，相关系数为0．9998～0．9999；检出限为1．09～2．06mg·^-1，定量限为3．25～6．46mg·^-1；异黄酮各异构体回收率为97．11％～103．31％；相对标准偏差（RSD，n=7）分别为2．03％～4．32％。该方法线性范围广，线性关系好，灵敏度和准确度高，适合于大砭及大豆制品中异黄酮含量的测定．测得吉林省261份普通大豆品种（系）中的异黄酬含量范吲1．46～4．97mg·g^-1，超过4mg·g^-1的品种（系）有23个，占测定品种（系）总数的8．81％。

A HPLC method tor rapid determination of isoflavones from soybean is presented. The isoflavones were extracted by 80% methanol and followed hydrolyzing at 100℃ to complete conversion of the acetyl and malonyl forms of isoflavones to β-glucoside and aglucone forms. The isoflavones were separated on a C18 short column using gradient elution;column oven temperature,40℃ ;the diode array detector monitoring wavelength ,260 nm. The peak area of isoflavones versus their coneentration had a good linearity（ eorrelation coefficient were 0. 9998-0. 9999）in the range of 50-1000 μmol·L^-1, Limits of detection（LOD） were in the range of 1.09- 2.06 mg·L^-1, limits of quantification （LOQ） were in the range of 3.25- 6.46mg·L^-1 The recoveries of standard, spiking of isoflavones were in the range of 97. 11% 103.31%. The relative standard deviation（ RSD, n = 7 ） were in the range of 2.03% -4.32%. The linearity of the method was good in a wide range. This method has high analytic sensitivity and accuracy,and was suitable for determination of isoflavones in soybean and other cereal products. The isoflavone content in 261 Jilin province normal soybean varieties（lines） ranged from 1.46 mg·g^-1 to 4.97 mg·g^-1. The isoflavone eontent of 23 varieties （lines） soybean exceeded 4 mg·g^-1 The research results indicated that the content range and characteristics of the soybean oligosaeeharides in Jilin province and lay a foundation for the further research of soybean quality breeding and processing anti utilization of soybean isotlavone.